Human IDH1 ELISA Kit0 Publications

Catalog number: KE00060



96 T


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Product name:
Human IDH1 ELISA Kit

1 X 96 well plate

Sample type:
Serum, Plasma, Cell lysates

Assay type:

3.3 pg/mL

62.5-4000 pg/mL

Reacted Species:

Tested applications:
Sandwich ELISA

Sample TypeAverageRange
Human plasma 98% 77%-125%
Cell lysates 96% 76%-128%

Samplenmean (pg/mL)SDCV%
1 20 3,400.2 112.2 3.3
2 20 726.7 19.2 2.6
3 20 176.0 5.4 3.0

Samplenmean (pg/mL)SDCV%
1 24 3,479.4 96.0 2.8
2 24 719.6 20.2 2.8
3 24 185.8 5.2 2.8

Product overview:
KE00060 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IDH1 ELISA kit is to be used to detect and quantify protein levels of endogenous IDH1. The assay recognizes human IDH1. A polyclonal antibody specific for IDH1 has been pre-coated onto the microwells. The IDH1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for IDH1 is added to detect the captured IDH1 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

IDCD, IDH, IDH1, IDP, IDPC, NADP(+) specific ICDH, Oxalosuccinate decarboxylase, PICD

IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. IDH1 may be a tumor suppressor that, when mutationally inactivated, contributes to tumorigenesis in part through induction of the HIF-1 pathway(PMID: 19359588). It is a common feature of a major subset of primary human brain cancers. IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homodimers and 50% heterodimers present in the tumor cells(PMID:21079649).

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