Human IL15 ELISA Kit0 Publications

Catalog number: KE00102

规格

单价

96 T

¥2600

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Overview


Product name:
Human IL15 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:
Sandwich

Sensitivity:
1.9 pg/mL

Range:
3.9 - 250 pg/mL

Reacted Species:
Human

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Cell culture supernatants 93% 72%-112%
Human plasma 104% 92%-116%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 20.9 0.9 4.1
2 20 81.8 4.2 5.1
3 20 309.1 15.5 5.0

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 21.4 1.5 7.2
2 24 77.4 5.2 6.8
3 24 274.4 23.9 8.7

Product overview:
KE00102 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL15 ELISA kit is to be used to detect and quantify protein levels of endogenous IL15. The assay recognizes human IL15. A monoclonal antibody specific for IL15 has been pre-coated onto the microwells. The IL15 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for IL15 is added to detect the captured IL15 protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
IL 15, IL15, IL-15, interleukin 15, interleukin-15
Background

Interleukin 15 (IL15) is a cytokine important for the development, maturation, and function of many cells of the immune system including NK, NKT, gammadeltaT, and CD8(+) T cells. Its expression affects homeostasis and function of B cells through NK cell-derived IFNG. IL-15 binds a trimeric IL-15 receptor (IL-15R) complex formed by a specific receptor chain (IL-15Rα) combined with theβandγchains of the IL-2R, acting as signal transducing components. High levels of IL15 expression have been associated with the pathogenesis of autoimmune and inflammatory diseases like Crohn’s disease, psoriasis, leukemias, rheumatoid arthritis (RA) and graft rejection, resulting in the IL15 system being a target of interest for the treatment of these diseases


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