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IBA1 Polyclonal antibody

IBA1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 26177-1-AP

Print datasheet

Synonyms

AIF 1, AIF1, G1, IBA1, IRT 1, Protein G1

验证数据展示

  • WB analysis of THP-1 using 26177-1-AP

    WB analysis of THP-1 using 26177-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 26177-1-AP (IBA1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 26177-1-AP

    IHC staining of rat brain using 26177-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26177-1-AP (IBA1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 26177-1-AP

    IHC staining of rat brain using 26177-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26177-1-AP (IBA1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inTHP-1 cells
Positive IHC detected inrat brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:400-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26177-1-AP is IBA1 Fusion Protein expressed in E. coli.

Tested Applications IHC, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Immunogen IBA1 fusion protein Ag24000 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name allograft inflammatory factor 1
Synonyms AIF 1, AIF1, G1, IBA1, IRT 1, Protein G1
Calculated Molecular Weight 17 kDa
GenBank Accession NumberBC009474
Gene Symbol AIF1
Gene ID (NCBI) 199
ENSEMBL Gene IDENSG00000204472
RRIDAB_2880412
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP55008
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.

实验方案

Product Specific Protocols
WB protocol for IBA1 antibody 26177-1-APDownload protocol
IHC protocol for IBA1 antibody 26177-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,ratWB,IF

Toxicon

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