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IBA1 Recombinant antibody

IBA1 Recombinant Antibody for FC, IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

FC, IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

4D5

Cat No : 81728-1-RR

Print datasheet

Synonyms

AIF 1, AIF1, G1, IBA1, IRT 1, Protein G1

验证数据展示

  • WB analysis of THP-1 using 81728-1-RR

    WB analysis of THP-1 using 81728-1-RR

    THP-1 cells were subjected to SDS PAGE followed by western blot with 81728-1-RR (IBA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 81728-1-RR

    IHC staining of rat brain using 81728-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 81728-1-RR

    IHC staining of rat brain using 81728-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 81728-1-RR

    IHC staining of rat brain using 81728-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat brain using 81728-1-RR

    IF Staining of rat brain using 81728-1-RR

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of rat brain using 81728-1-RR

    IF Staining of rat brain using 81728-1-RR

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of THP-1 using 81728-1-RR

    FC experiment of THP-1 using 81728-1-RR

    1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human IBA1 (81728-1-RR, Clone:4D5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inTHP-1 cells
Positive IHC detected inrat brain tissue, mouse brain tissue, human liver tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inrat brain tissue
Positive FC detected inTHP-1 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:250-1:1000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81728-1-RR targets IBA1 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC, IF-P, IHC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen IBA1 fusion protein Ag1363 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name allograft inflammatory factor 1
Synonyms AIF 1, AIF1, G1, IBA1, IRT 1, Protein G1
Calculated Molecular Weight 17 kDa
Observed Molecular Weight17 kDa
GenBank Accession NumberBC009474
Gene Symbol AIF1
Gene ID (NCBI) 199
ENSEMBL Gene IDENSG00000204472
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55008
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.

实验方案

Product Specific Protocols
WB protocol for IBA1 antibody 81728-1-RRDownload protocol
IHC protocol for IBA1 antibody 81728-1-RRDownload protocol
IF protocol for IBA1 antibody 81728-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols