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CoraLite®555-conjugated IBA1 Recombinant antibody

IBA1 Recombinant Antibody for FC (Intra), IF-P

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

FC (Intra), IF-P

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

4D5

Cat No : CL555-81728

Print datasheet

Synonyms

AIF 1, AIF1, G1, IBA1, IRT 1, Protein G1

验证数据展示

  • IF Staining of rat brain using CL555-81728

    IF Staining of rat brain using CL555-81728

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite®555 IBA1 antibody (CL555-81728, Clone: 4D5 ) at dilution of 1:200.

  • IF Staining of rat brain using CL555-81728

    IF Staining of rat brain using CL555-81728

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using CoraLite®555 IBA1 antibody (CL555-81728, Clone: 4D5 ) at dilution of 1:200.

  • FC experiment of THP-1 using CL555-81728

    FC experiment of THP-1 using CL555-81728

    1X10^6 THP-1 cells were intracellularly stained with 0.8 ug CoraLite®555 Anti-Human IBA1 (CL555-81728, Clone:4D5) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inrat brain tissue
Positive FC detected inTHP-1 cells
For other applications, we recommend the unconjugated version of this antibody, 81728-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-81728 targets IBA1 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra), IF-P
Tested Reactivity Human, mouse, rat
Immunogen IBA1 fusion protein Ag1363 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name allograft inflammatory factor 1
Synonyms AIF 1, AIF1, G1, IBA1, IRT 1, Protein G1
Calculated Molecular Weight 17 kDa
Observed Molecular Weight17 kDa
GenBank Accession NumberBC009474
Gene Symbol AIF1
Gene ID (NCBI) 199
ENSEMBL Gene IDENSG00000204472
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55008
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.

实验方案

Product Specific Protocols
IF protocol for CL555 IBA1 antibody CL555-81728Download protocol
FC protocol for CL555 IBA1 antibody CL555-81728Download protocol
Standard Protocols
Click here to view our Standard Protocols