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ICAM2 Polyclonal antibody 2 Publications

Rabbit Polyclonal| Catalog number: 10121-2-AP

Featured Product KD/KO validated

浓度: 250 μg/ml

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Applications

Tested Applications:
IHC, WB,ELISA
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Cited Applications:
IHC, WB


Images See all

WB analysis of Jurkat using 10121-2-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 10121-2-AP (ICAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

IHC staining of human breast cancer using 10121-2-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10121-2-AP (ICAM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IHC staining of human breast cancer using 10121-2-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10121-2-AP (ICAM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Species specificity:
human
Positive WB detected in:
Jurkat cells, HEK-293 cells
Positive IHC detected in:
human breast cancer tissue, human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:500

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ICAM2 fusion protein Ag0165
Full name:
intercellular adhesion molecule 2
Calculated molecular weight:
31 kDa
Observed molecular weight:
55-80 kDa
GenBank accession number:
BC003097
Gene ID (NCBI):
3384
Gene symbol
ICAM2
Synonyms
CD102, ICAM 2, ICAM2

Background

ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. ICAM2 has six N-linked glycosylation sites at amino acids (asparagines) 47, 82, 105, 153, 178 and 187. This antibody got 55-80 kDa in western blotting maybe due to glycosylation.

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