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ICAM2 Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 10121-2-AP

Featured Product KD/KO validated

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浓度: 250μg/ml

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Tested Applications

Applications:
WB, IHC, ELISA
Application not tested? Please see our Guarantee


Images See all

ICAM2 Antibody WB Jurkat cells 10121-2-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 10121-2-AP(ICAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

ICAM2 Antibody IHC human breast cancer tissue 10121-2-AP

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10121-2-AP( ICAM2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

ICAM2 Antibody IHC human breast cancer tissue 10121-2-AP

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10121-2-AP( ICAM2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:
human, rat, mouse
Positive WB detected in:
Jurkat cells, HEK-293 cells
Positive IHC detected in:
human breast cancer tissue, human lung cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:1000
IHC : 1:50-1:500

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
ICAM2 fusion protein Ag0165
Full name:
intercellular adhesion molecule 2
Calculated molecular weight:
31 kDa
Observed molecular weight:
55-80 kDa
GenBank accession number:
BC003097
Gene ID (NCBI):
3384
Gene symbol
ICAM2
Synonyms
CD102, ICAM 2, ICAM2

Background

ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. ICAM2 has six N-linked glycosylation sites at amino acids (asparagines) 47, 82, 105, 153, 178 and 187. This antibody got 55-80 kDa in western blotting maybe due to glycosylation.


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