ICAM2 Antibody 10121-2-AP

Tested Applications

Applications:

WB, IHC, ELISA
Application not tested? Please see our Guarantee

ICAM2 Antibody WB Jurkat cells 10121-2-AP

Jurkat cells were subjected to SDS PAGE followed by western blot with 10121-2-AP(ICAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

ICAM2 Antibody IHC human breast cancer tissue 10121-2-AP

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10121-2-AP( ICAM2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10121-2-AP( ICAM2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Species specificity:

human, rat, mouse

Positive WB detected in:

Jurkat cells, HEK-293 cells

Positive IHC detected in:

human breast cancer tissue, human lung cancer tissue

Recommended dilution:

WB : 1:500-1:1000

IHC : 1:50-1:500

Product Information

Source:

Rabbit

Purification method:

Antigen affinity purification

Isotype:

IgG

Storage:

PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20^oC. Aliquoting is unnecessary for -20^oC storage.

Immunogen Information

Immunogen:

ICAM2 fusion protein Ag0165

Full name:

intercellular adhesion molecule 2

Calculated molecular weight:

31 kDa

Observed molecular weight:

55-80 kDa

GenBank accession number:

BC003097

Gene ID (NCBI):

3384

Gene symbol

ICAM2

Synonyms

CD102, ICAM 2, ICAM2

Background

ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. ICAM2 has six N-linked glycosylation sites at amino acids (asparagines) 47, 82, 105, 153, 178 and 187. This antibody got 55-80 kDa in western blotting maybe due to glycosylation.

Showing 1- [2] of [2] Publications

Author:		Yoon Karina Jin KJ
Pubmed ID:		18978946
Journal:		PLoS One
Title:		ICAM-2 expression mediates a membrane-actin link, confers a nonmetastatic phenotype and reflects favorable tumor stage or histology in neuroblastoma.
Application:		WB,IHC
KD validated
Species:		human

Author:		Yu Dong
Pubmed ID:		24457076
Journal:		Hum Pathol
Title:		Intercellular adhesion molecule 1/2 and E-selectin in plasma cell mastitis: immunohistochemical study of 35 cases.
Application:		IHC

Species:		human

WB Figures

ICAM2 Antibody WB HEK-293 cells 10121-2-AP

HEK-293 cells were subjected to SDS PAGE followed by western blot with 10121-2-AP(ICAM2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours

Jurkat cells were subjected to SDS PAGE followed by western blot with 10121-2-AP(ICAM2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours

IHC Figures

ICAM2 Antibody IHC human lung cancer tissue 10121-2-AP

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 10121-2-AP( ICAM2 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 10121-2-AP( ICAM2 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

规格	单价
50 μl	￥980
100 μl	￥1800
150 μl	￥2500

ICAM2 Antibody 2 Publications

Rabbit Polyclonal| Catalog number: 10121-2-AP