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CoraLite® Plus 488-conjugated IDE Monoclonal antibody

IDE Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Mouse, Rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3E10G1

Cat No : CL488-67106

Print datasheet

Synonyms

Abeta degrading protease, FLJ35968, IDE, insulin degrading enzyme, Insulin protease, Insulinase, INSULYSIN

验证数据展示

  • IF Staining of A549 using CL488-67106

    IF Staining of A549 using CL488-67106

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite® Plus 488 IDE antibody (CL488-67106, Clone: 3E10G1 ) at dilution of 1:200.

  • FC experiment of A549 using CL488-67106

    FC experiment of A549 using CL488-67106

    1X10^6 A549 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human IDE (CL488-67106, Clone:3E10G1) (red), or 0.4 ug Mouse IgG2b Isotype Control (CL488-66360-3, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF detected inA549 cells
Positive FC detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 67106-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67106 targets IDE in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, Mouse, Rat
Immunogen IDE fusion protein Ag17161 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name Abeta degrading protease
Synonyms Abeta degrading protease, FLJ35968, IDE, insulin degrading enzyme, Insulin protease, Insulinase, INSULYSIN
Calculated Molecular Weight 1019 aa, 118 kDa
Observed Molecular Weight 100-120 kDa
GenBank Accession NumberBC096336
Gene Symbol IDE
Gene ID (NCBI) 3416
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP14735
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IDE(INS-degrading enzyme) belongs to the peptidase M16 family. It is also known as insulysin and is a 110-kD neutral metallopeptidase that can degrade a number of peptides, including INS and beta-amyloid(PMID:9830016). The reduction of IDE expression may be a risk factor for AD, and that IDE may interact with APOE status to affect beta-amyloid metabolism(PMID:12507914). It can exsit as a homodimer and form higher oligomers(PMID:17613531).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 IDE antibody CL488-67106Download protocol
FC protocol for CL Plus 488 IDE antibody CL488-67106Download protocol
Standard Protocols
Click here to view our Standard Protocols