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IFNGR1 Monoclonal antibody

IFNGR1 Monoclonal Antibody for WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB,ELISA

Conjugate

Unconjugated

CloneNo.

1H3A4

Cat No : 66704-1-Ig

Print datasheet

Synonyms

CD119, CDw119, FLJ45734, IFN gamma R1, IFN gamma receptor 1, IFNGR, IFNGR1, interferon gamma receptor 1

验证数据展示

  • WB analysis of HeLa using 66704-1-Ig

    WB analysis of HeLa using 66704-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 66704-1-Ig (IFNGR1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, T-47D cells, Jurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66704-1-Ig targets IFNGR1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB,ELISA
Tested Reactivity Human
Immunogen IFNGR1 fusion protein Ag27085 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name interferon gamma receptor 1
Synonyms CD119, CDw119, FLJ45734, IFN gamma R1, IFN gamma receptor 1, IFNGR, IFNGR1, interferon gamma receptor 1
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 90 kDa
GenBank Accession NumberBC005333
Gene Symbol IFNGR1
Gene ID (NCBI) 3459
RRIDAB_2882056
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15260
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Interferon-gamam (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 (CD119) and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit. Two subunits bind one IFN-γ dimer. Defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. (PMID: 17981204; 946248; 10888113)

实验方案

Product Specific Protocols
WB protocol for IFNGR1 antibody 66704-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols