IFT88 Recombinant antibody
IFT88 Recombinant Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, mouse, rat
Applications
IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
230074F8
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, NIH/3T3 cells, human testis tissue, mouse testis tissue, rat testis tissue |
Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82886-1-RR targets IFT88 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
Tested Applications | IHC, WB, ELISA |
Tested Reactivity | Human, mouse, rat |
Immunogen | IFT88 fusion protein Ag4980 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | intraflagellar transport 88 homolog (Chlamydomonas) |
Synonyms | D13S1056E, DAF19, hTg737, IFT88, RP11 172H24.2, TG737, TPR repeat protein 10, TTC10 |
Calculated Molecular Weight | 94 kDa |
Observed Molecular Weight | 88-95 kDa |
GenBank Accession Number | BC030776 |
Gene Symbol | IFT88 |
Gene ID (NCBI) | 8100 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q13099 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.
实验方案
Product Specific Protocols | |
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WB protocol for IFT88 antibody 82886-1-RR | Download protocol |
IHC protocol for IFT88 antibody 82886-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |