验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HSC-T6 cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells, HepG2 cells, K-562 cells |
Planning an IHC experiment? We recommend our IHCeasy IGF2BP3 Ready-To-Use IHC Kit. IGF2BP3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
66526-1-Ig targets IGF2BP3 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | IGF2BP3 fusion protein Ag6349 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | insulin-like growth factor 2 mRNA binding protein 3 |
| 别名 | hKOC, IGF II mRNA binding protein 3, IGF2 mRNA binding protein 3, IGF2BP3, IMP 3, IMP3, KOC1, VICKZ family member 3, VICKZ3 |
| 计算分子量 | 64 kDa |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | BC065269 |
| 基因名称 | IGF2BP3 |
| Gene ID (NCBI) | 10643 |
| RRID | AB_2881889 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | O00425 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IGF2BP3, also named as IMP3, KOC1 and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IGF2BP3 antibody 66526-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
