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验证数据展示

  • WB analysis using 66526-1-Ig

    WB analysis using 66526-1-Ig

    Various cells were subjected to SDS PAGE followed by western blot with 66526-1-Ig (IGF2BP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 66526-1-Ig

    WB analysis using 66526-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66526-1-Ig (IGF2BP3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

IGF2BP3 Monoclonal antibody

IGF2BP3 Monoclonal Antibody for WB, ELISA
Cat No. 66526-1-Ig
CloneNo. 2C10B11
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat and More (1)

Applications

WB, ELISA

Synonyms

hKOC, IGF II mRNA binding protein 3, IGF2 mRNA binding protein 3, IGF2BP3, IMP 3, IMP3, KOC1, VICKZ family member 3, VICKZ3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, NIH/3T3 cells, HEK-293 cells, Jurkat cells, HepG2 cells, K-562 cells
Planning an IHC experiment? We recommend our IHCeasy IGF2BP3 Ready-To-Use IHC Kit. IGF2BP3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

66526-1-Ig targets IGF2BP3 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Immunogen IGF2BP3 fusion protein Ag6349 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name insulin-like growth factor 2 mRNA binding protein 3
Synonyms hKOC, IGF II mRNA binding protein 3, IGF2 mRNA binding protein 3, IGF2BP3, IMP 3, IMP3, KOC1, VICKZ family member 3, VICKZ3
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC065269
Gene Symbol IGF2BP3
Gene ID (NCBI) 10643
RRIDAB_2881889
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00425
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IGF2BP3, also named as IMP3, KOC1 and VICKZ3, belongs to the RRM IMP/VICKZ family. It is one of the RNA binding proteins involved in mRNA localization and translational control. IGF2BP3 is expressed during embryogenesis, as well as in some malignant tumors. It can be used as an independent prognostic factor for osteosarcoma.

实验方案

Product Specific Protocols
WB protocol for IGF2BP3 antibody 66526-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell

An in vitro system to silence mitochondrial gene expression.

Authors - Luis Daniel Cruz-Zaragoza
View Article