IHCeasy ECD Ready-To-Use IHC Kit

ECD Ready-to-use reagent kit for IHC.

Reactivity

Human, Mouse, Rat

Sample Type

FFPE tissue

Cat No : KHC1501

Synonyms

ECD, GCR2, HSGT1, Protein ecdysoneless homolog, Protein SGT1, SGT1, Suppressor of GCR2



Product Information

KHC1501 is a ready-to-use IHC kit for staining of ECD. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy ECD Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Mouse Monoclonal
Secondary antibody type Polymer-HRP-Goat anti-Mouse
Reactivity Human, Mouse, Rat

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

The human suppressor of GCR two (hSGT1) was isolated by its ability to complement the growth defect of the gcr2 mutant of Saccharomyces cerevisiae. Further work confirmed that the rescue of growth was associated with recovery of glycolytic enzyme activities of gcr2, and that hSGT1 did not complement the growth defect of a gcr1 mutant. A hybrid protein comprising hSgt1p and the DNA-binding domain of Gal4p (GBD) activated a GAL1-lacZ reporter gene fusion, suggesting that the cloned gene may be a transcriptional activator. Two-hybrid experiments in yeast also indicate that hSgt1p interacts with Gcr1p. Northern analysis showed that hSGT1 is highly expressed in muscle and heart.

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms ECD, GCR2, HSGT1, Protein ecdysoneless homolog, Protein SGT1, SGT1, Suppressor of GCR2