• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

IL-12RB1 Polyclonal antibody

IL-12RB1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

IHC, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 13287-1-AP

Print datasheet

Synonyms

CD212, IL 12 receptor beta component, IL 12 receptor subunit beta 1, IL 12R beta 1, IL 12R BETA1, IL 12R subunit beta 1, IL 12RB1, IL-12 R beta 1, IL12R, IL12RB, IL12RB1, IL-12RB1

验证数据展示

  • WB analysis of K-562 using 13287-1-AP

    WB analysis of K-562 using 13287-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 13287-1-AP (IL12RB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 13287-1-AP

    IHC staining of human tonsillitis using 13287-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13287-1-AP (IL12RB1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of Jurkat using 13287-1-AP

    WB analysis of Jurkat using 13287-1-AP

    WB result of IL-12RB1 antibody (13287-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-IL-12RB1 transfected Jurkat cells.

经过测试的应用

Positive WB detected inK-562 cells, Jurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13287-1-AP targets IL-12RB1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB, IP, IF, IHC
Tested Reactivity human
Cited Reactivityhuman, mouse, monkey
Immunogen IL-12RB1 fusion protein Ag3983 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interleukin 12 receptor, beta 1
Synonyms CD212, IL 12 receptor beta component, IL 12 receptor subunit beta 1, IL 12R beta 1, IL 12R BETA1, IL 12R subunit beta 1, IL 12RB1, IL-12 R beta 1, IL12R, IL12RB, IL12RB1, IL-12RB1
Calculated Molecular Weight 662 aa, 73 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC029121
Gene Symbol IL12RB1
Gene ID (NCBI) 3594
RRIDAB_2124039
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP42701
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL12RB1, also named as Interleukin-12 receptor subunit beta-1 or CD212, is a 662 amino acid protein, which contains 5 fibronectin type-III domains and belongs to the type I cytokine receptor family. Type 2 subfamily. IL12RB1 localizes in the membrane and functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.

实验方案

Product Specific Protocols
WB protocol for IL-12RB1 antibody 13287-1-APDownload protocol
IHC protocol for IL-12RB1 antibody 13287-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human, monkeyWB, IP, IF

J Immunol

Inflammatory signals direct expression of human IL12RB1 into multiple distinct isoforms.

Authors - Ford Nicole R NR
View Article
mouseIHC,IF

Front Med (Lausanne)

Serum IL-12p40: A novel biomarker for early prediction of minimal change disease relapse following glucocorticoids therapy

Authors - Mengqiu Bai
View Article