ST2 Monoclonal antibody
ST2 Monoclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat, pig
Applications
FC, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
CloneNo.
7A2A7
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, fetal human brain tissue, SGC-7901 cells, pig brain tissue, mouse brain tissue, HSC-T6 cells, ROS1728 cells, PC-12 cells, rat brain tissue, rat cerebellum tissue |
Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | HEK-293 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Flow Cytometry (FC) | FC : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
60112-1-Ig targets ST2 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | FC, IHC, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse, rat, pig |
Cited Reactivity | human, mouse, pig |
Immunogen | ST2 fusion protein Ag2555 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | interleukin 1 receptor-like 1 |
Synonyms | DER4, FIT 1, IL1RL1, IL-1RL1, IL-33R, interleukin 1 receptor like 1, Protein ST2, ST2, ST2L, ST2V, T1 |
Calculated Molecular Weight | 63 kDa, 37 kDa, 30 kDa |
Observed Molecular Weight | 63 kDa |
GenBank Accession Number | BC030975 |
Gene Symbol | IL1RL1 |
Gene ID (NCBI) | 9173 |
RRID | AB_2264785 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Caprylic acid/ammonium sulfate precipitation |
UNIPROT ID | Q01638 |
Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ST2 antibody 60112-1-Ig | Download protocol |
IHC protocol for ST2 antibody 60112-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Immunity Interleukin-33 Promotes Serotonin Release from Enterochromaffin Cells for Intestinal Homeostasis. | ||
Sci Rep Dysfunctional EAT thickness may promote maladaptive heart remodeling in CVD patients through the ST2-IL33 system, directly related to EPAC protein expression. | ||
Sci Rep Fatty acid-binding protein 5 limits ILC2-mediated allergic lung inflammation in a murine asthma model. | ||
Cancer Biomark Targeting IL-17alpha to promote anti-PD-1 therapy effect by screening the tumor immune microenvironment in a mouse oral carcinogenesis model | ||
Am J Physiol Renal Physiol INTRA-RENAL MODULATION OF NF-κB ACTIVITY ATTENUATES CARDIAC INJURY IN A SWINE MODEL OF CKD: A RENAL-CARDIO AXIS. |