IRF5 Polyclonal antibody
IRF5 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
FC, IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | THP-1 cells, SH-SY5Y cells, Jurkat cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human prostate cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
10547-1-AP targets IRF5 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IP, IF, FC, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, brovine, sheep |
Immunogen | IRF5 fusion protein Ag0841 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | interferon regulatory factor 5 |
Synonyms | interferon regulatory factor 5, IRF 5, IRF5, SLEB10 |
Calculated Molecular Weight | 56 kDa |
Observed Molecular Weight | 56 kDa |
GenBank Accession Number | BC004201 |
Gene Symbol | IRF5 |
Gene ID (NCBI) | 3663 |
RRID | AB_2280474 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q13568 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for IRF5 antibody 10547-1-AP | Download protocol |
IHC protocol for IRF5 antibody 10547-1-AP | Download protocol |
IF protocol for IRF5 antibody 10547-1-AP | Download protocol |
IP protocol for IRF5 antibody 10547-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
J Immunother Cancer Ccl3 enhances docetaxel chemosensitivity in breast cancer by triggering proinflammatory macrophage polarization. | ||
Am J Transplant Characterization of Transfusion-Elicited Acute Antibody-Mediated Rejection in a Rat Model of Kidney Transplantation. | ||
J Control Release PEGylation of interleukin-10 improves the pharmacokinetic profile and enhances the antifibrotic effectivity in CCl?-induced fibrogenesis in mice. | ||
Br J Pharmacol Ibuprofen and diclofenac treatments reduce proliferation of pancreatic acinar cells upon inflammatory injury and mitogenic stimulation. | ||
Biochem Pharmacol Quercetin ameliorates kidney injury and fibrosis by modulating M1/M2 macrophage polarization. |