验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, human placenta tissue, U2OS cells |
| Positive IP detected in | human placenta tissue |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ITGA5 Ready-To-Use IHC Kit. ITGA5 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
27224-1-AP targets Integrin Alpha 5 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IP, IHC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | Integrin Alpha 5 fusion protein Ag26098 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | integrin, alpha 5 (fibronectin receptor, alpha polypeptide) |
| Synonyms | CD49e, FNRA, Integrin alpha 5, Integrin alpha F, ITGA5, VLA 5, VLA5A |
| Calculated Molecular Weight | 115 kDa |
| Observed Molecular Weight | 135-150 kDa |
| GenBank Accession Number | NM_002205 |
| Gene Symbol | Integrin alpha 5 |
| Gene ID (NCBI) | 3678 |
| RRID | AB_2880807 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Integrin Alpha 5 antibody 27224-1-AP | Download protocol |
| IHC protocol for Integrin Alpha 5 antibody 27224-1-AP | Download protocol |
| IP protocol for Integrin Alpha 5 antibody 27224-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Transl Hepatol A Prognostic Nomogram for Hepatocellular Carcinoma Based on Wound Healing and Immune Checkpoint Genes | ||
Mol Cancer ZNF460-mediated circRPPH1 promotes TNBC progression through ITGA5-induced FAK/PI3K/AKT activation in a ceRNA manner | ||
Biomater Res Targeting Reprogrammed Cancer-Associated Fibroblasts with Engineered Mesenchymal Stem Cell Extracellular Vesicles for Pancreatic Cancer Treatment
|
