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  • Featured Product
  • KD/KO Validated

Integrin Alpha 5 Polyclonal antibody

Integrin Alpha 5 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 27224-1-AP

Print datasheet

Synonyms

CD49e, FNRA, Integrin alpha 5, Integrin alpha F, ITGA5, VLA 5, VLA5A

验证数据展示

  • WB analysis using 27224-1-AP

    WB analysis using 27224-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27224-1-AP (Integrin alpha-5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 27224-1-AP

    WB analysis of HeLa using 27224-1-AP

    WB result of Integrin alpha-5 antibody (27224-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Integrin alpha-5 transfected HeLa cells.

  • IP experiment of human placenta using 27224-1-AP

    IP experiment of human placenta using 27224-1-AP

    IP result of anti-Integrin Alpha 5 (IP:27224-1-AP, 4ug; Detection:27224-1-AP 1:1000) with human placenta tissue lysate 2600 ug.

  • IHC staining of human placenta using 27224-1-AP

    IHC staining of human placenta using 27224-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27224-1-AP (Integrin alpha-5 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with ITGA5 antibody included
  • IHC staining of human placenta using 27224-1-AP

    IHC staining of human placenta using 27224-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27224-1-AP (Integrin alpha-5 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with ITGA5 antibody included

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, human placenta tissue, U2OS cells
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ITGA5 Ready-To-Use IHC Kit. ITGA5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 27224-1-AP is Integrin Alpha 5 Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, WB, ELISA
Tested Reactivity human
Immunogen Integrin Alpha 5 fusion protein Ag26098 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
Synonyms CD49e, FNRA, Integrin alpha 5, Integrin alpha F, ITGA5, VLA 5, VLA5A
Calculated Molecular Weight 115 kDa
Observed Molecular Weight 135-150 kDa
GenBank Accession NumberNM_002205
Gene Symbol ITGA5
Gene ID (NCBI) 3678
RRIDAB_2880807
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Integrin Alpha 5 antibody 27224-1-APDownload protocol
IHC protocol for Integrin Alpha 5 antibody 27224-1-APDownload protocol
IP protocol for Integrin Alpha 5 antibody 27224-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols