验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, A2780 cells |
| Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Planning an IHC experiment? We recommend our IHCeasy ITGB6 Ready-To-Use IHC Kit. ITGB6 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19695-1-AP targets Integrin beta-6-Specific in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP, IF, IHC |
| 经测试反应性 | human |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | integrin, beta 6 |
| 别名 | Integrin beta 6, Integrin beta-6-Specific, integrin, beta 6, ITGB6 |
| 计算分子量 | 86 kDa |
| 观测分子量 | 97 kDa |
| GenBank蛋白编号 | NM_000888 |
| 基因名称 | Integrin beta 6 |
| Gene ID (NCBI) | 3694 |
| RRID | AB_10644138 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P18564 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ITGB6 belongs to the integrin beta chain family. ITGB6 is a receptor for fibronectin and cytotactin. It recognizes the sequence R-G-D in its ligands. Internalisation of integrin alpha-V/beta-6 via clathrin-mediated endocytosis promotes carcinoma cell invasion. The antibody is specific to ITGB6.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Integrin beta-6-Specific antibody 19695-1-AP | Download protocol |
| IHC protocol for Integrin beta-6-Specific antibody 19695-1-AP | Download protocol |
| IF protocol for Integrin beta-6-Specific antibody 19695-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Glia αV integrins in Schwann cells promote attachment to axons, but are dispensable in vivo. | ||
Cell Biosci Protein expression of eIF4E and integrin αvβ6 in colon cancer can predict clinical significance, reveal their correlation and imply possible mechanism of interaction. | ||
J Exp Clin Cancer Res Stanniocalcin 1 promotes metastasis, lipid metabolism and cisplatin chemoresistance via the FOXC2/ITGB6 signaling axis in ovarian cancer. | ||
Mol Cell Endocrinol Integrin β6 mediates epithelial-mesenchymal transition in diabetic kidney disease | ||
Nat Commun A circular RNA activated by TGFβ promotes tumor metastasis through enhancing IGF2BP3-mediated PDPN mRNA stability |
