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mouse Icam-1 Polyclonal antibody

Icam-1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (2)

Applications

IF, IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 10020-1-AP

Print datasheet

Synonyms

CD54, ICAM 1, Icam1, Icam-1, Ly 47, MALA 2



经过测试的应用

Positive WB detected inmouse brain tissue, mouse spleen tissue, mouse liver tissue
Positive IHC detected inmouse lung tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse liver tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:3200-1:12800
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10020-1-AP targets Icam-1 in WB, IHC, IF, FC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications IF, IHC, WB, ELISA
Cited Applications FC, IF, IHC, WB
Tested Reactivity mouse, rat
Cited Reactivitymouse, rat, hamster, bovine
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name intercellular adhesion molecule 1
Synonyms CD54, ICAM 1, Icam1, Icam-1, Ly 47, MALA 2
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 95-100 kDa
GenBank Accession NumberNM_010493
Gene Symbol Icam1
Gene ID (NCBI) 15894
RRIDAB_2121773
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13597
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Where is ICAM-1 expressed?

Intercellular Adhesion Molecule 1 (ICAM-1), also known as Cluster of Differentiation 54 (CD54) is a transmembrane glycoprotein constitutively expressed at low levels in endothelial cells, pericytes and on some lymphocytes and monocytes1. It is located at the cytoplasmic membrane, with a large extracellular region of mainly hydrophobic amino acids joined to a small transmembrane region and a cytoplasmic tail. It has a molecular weight of 75 to 115 kDa depending on the level of glycosylation.

 What is the function of ICAM-1?

ICAM-1 is important in both innate and adaptive immune responses as an adhesion molecule. Although it is constitutively expressed, in the presence of pro-inflammatory cytokines such as TNFα the endothelial cells are activated and upregulate expression of ICAM-12. In blood vessels lined with endothelial cells, leukocytes that are rolling over the surface are able to bind to ICAM-1 and transmigrate through the endothelial barrier and into the tissue. The initial binding of the leukocytes to ICAM-1 causes a Ca2+ release that initiates endothelial cell contraction and weakening of the intercellular tight junctions3, 4. This protein can be used as an indicator of endothelial activation and of vascular inflammation.

 What is the role of ICAM-1 in disease?

Beyond the role in the immune response, ICAM-1 has also been identified as the target of attachment for the human rhinovirus, the cause of the common cold. Binding of the virus to ICAM-1 causes the viral capsid to uncoat and leads to release of the genetic material5.

  1.  Hubbard, A. K. & Rothlein, R. Intercellular adhesion molecule-1 (ICAM-1) expression and cell signaling cascades. Free Radic. Biol. Med. 28, 1379-86 (2000).

  2. Long, E. O. ICAM-1: getting a grip on leukocyte adhesion. J. Immunol. 186, 5021-3 (2011).

  3. Lawson, C. & Wolf, S. ICAM-1 signaling in endothelial cells. (2009).

  4. Lyck, R. & Enzmann, G. The physiological roles of ICAM-1 and ICAM-2 in neutrophil migration into tissues. Curr. Opin. Hematol. 22, 53-59 (2015).

  5. Xing, L., Casasnovas, J. M. & Cheng, R. H. Structural analysis of human rhinovirus complexed with ICAM-1 reveals the dynamics of receptor-mediated virus uncoating. J. Virol. 77, 6101-7 (2003).

实验方案

Product Specific Protocols
WB protocol for Icam-1 antibody 10020-1-APDownload protocol
IHC protocol for Icam-1 antibody 10020-1-APDownload protocol
IF protocol for Icam-1 antibody 10020-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Immunity

Toll-like receptor 4 signaling by follicular dendritic cells is pivotal for germinal center onset and affinity maturation.

Authors - Alexandre Garin
mouseIF

Nature

Mechanosensing by β1 integrin induces angiocrine signals for liver growth and survival.

Authors - Linda Lorenz
mouseIF

Gastroenterology

ECM1 Prevents Activation of Transforming Growth Factor β, Hepatic Stellate Cells, and Fibrogenesis in Mice.

Authors - Weiguo Fan

Hepatology

Angiocrine Wnt signaling controls liver growth and metabolic maturation in mice.

Authors - Thomas Leibing
mouseWB

Nat Commun

Reducing Akt2 in retinal pigment epithelial cells causes a compensatory increase in Akt1 and attenuates diabetic retinopathy

Authors - Haitao Liu
IF

EBioMedicine

Patient-derived melanoma organoid models facilitate the assessment of immunotherapies

Authors - Lingling Ou