Back

Icam-1 Polyclonal antibody 40 Publications

Rabbit Polyclonal| Catalog number: 10020-1-AP

Featured Product KD/KO validated

浓度: 1227 μg/ml

点击咨询在线客服

Applications

Tested Applications:
IHC, WB, ELISA
Application not tested? Please see our Guarantee


Cited Applications:
FC, IF, IHC, WB


Images See all

WB analysis of mouse brain using 10020-1-AP

mouse brain tissue were subjected to SDS PAGE followed by western blot with 10020-1-AP (Icam-1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

IHC staining of mouse liver using 10020-1-AP

Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10020-1-AP (Icam-1 antibody) at dilution of 1:200 (under 10x lens).

IHC staining of mouse liver using 10020-1-AP

Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10020-1-AP (Icam-1 antibody) at dilution of 1:200 (under 40x lens).

Species specificity:
mouse, rat, bovine, Chinese hamster, hamster
Positive WB detected in:
mouse brain tissue, mouse spleen tissue, mouse liver tissue
Positive IHC detected in:
mouse liver tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:1000-1:4000
IHC : 1:20-1:200

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Recombinant Protein
Full name:
intercellular adhesion molecule 1
Calculated molecular weight:
59 kDa
Observed molecular weight:
95-100 kDa
GenBank accession number:
NM_010493
Gene ID (NCBI):
15894
Gene symbol
Icam1
Synonyms
CD54, ICAM 1, Icam1, Ly 47, MALA 2

Background

ICAM-1 (Intercellular Adhesion Molecule-1, CD54) is a glycosylated, transmembrane protein, a member of Ig super family. This protein involves in cell adhesion by interacting with a number of 2 integrin family members such as LFA-1 and Mac-1. Its expression is regulated by chemokines. This antibody is raised against mouse Icam-1 which is a cell surface sialoglycoprotein with an apparent molecular weight of 95-100 kDa (PMID: 10064885; 2571505).

Protocols

PRODUCT SPECIFIC PROTOCOLS

STANDARD PROTOCOLS

Click here to view our Standard Protocols

Back
to top