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Ins1 Polyclonal antibody

Ins1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 28588-1-AP

Print datasheet

Synonyms

Ins 1, Ins1, Ins2 rs1, insulin I, Insulin-1

验证数据展示

  • WB analysis using 28588-1-AP

    WB analysis using 28588-1-AP

    Rat pancreas tissue were subjected to SDS PAGE followed by western blot with 28588-1-AP (Insulin1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse pancreas using 28588-1-AP

    IHC staining of mouse pancreas using 28588-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28588-1-AP (Insulin1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 28588-1-AP

    IHC staining of mouse pancreas using 28588-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 28588-1-AP (Insulin1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inrat pancreas tissue
Positive IHC detected inmouse pancreas tissue, rat pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28588-1-AP targets Ins1 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications IHC, WB,ELISA
Tested Reactivity mouse, rat
Immunogen Ins1 fusion protein Ag29988 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name insulin I
Synonyms Ins 1, Ins1, Ins2 rs1, insulin I, Insulin-1
Calculated Molecular Weight 12 kDa
Observed Molecular Weight12 kDa
GenBank Accession NumberNM_008386
Gene Symbol Ins1
Gene ID (NCBI) 16333
RRIDAB_2881177
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP01325
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

insulin I is a peptide hormone that plays a vital role in the regulation of carbohydrate and lipid metabolism. The encoded precursor protein undergoes proteolytic cleavage to produce a disulfide-linked heterodimeric functional protein that is stored in secretory granules. An increase in blood glucose levels, among others, induces the release of insulin from the secretory granules. Mice deficient in the functional hormone encoded by this gene develop diabetes mellitus.

实验方案

Product Specific Protocols
WB protocol for Ins1 antibody 28588-1-APDownload protocol
IHC protocol for Ins1 antibody 28588-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols