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KIF14 Polyclonal antibody

KIF14 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 26000-1-AP

Print datasheet

Synonyms

KIAA0042, KIF14, kinesin family member 14, Kinesin like protein KIF14

验证数据展示

  • WB analysis of T-47D using 26000-1-AP

    WB analysis of T-47D using 26000-1-AP

    T-47D cells were subjected to SDS PAGE followed by western blot with 26000-1-AP (KIF14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 26000-1-AP

    IHC staining of human prostate cancer using 26000-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26000-1-AP (KIF14 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HEK-293T using 26000-1-AP

    IP experiment of HEK-293T using 26000-1-AP

    IP result of anti-KIF14 (IP:26000-1-AP, 4ug; Detection:26000-1-AP 1:300) with HEK-293T cells lysate 4000 ug.

  • IHC staining of human prostate cancer using 26000-1-AP

    IHC staining of human prostate cancer using 26000-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 26000-1-AP (KIF14 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inT-47D cells, HEK-293T cells, HeLa cells
Positive IP detected inHEK-293T cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 26000-1-AP is KIF14 Fusion Protein expressed in E. coli.

Tested Applications IHC, IP, WB,ELISA
Tested Reactivity human
Immunogen KIF14 fusion protein Ag23219 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kinesin family member 14
Synonyms KIAA0042, KIF14, kinesin family member 14, Kinesin like protein KIF14
GenBank Accession NumberBC113742
Gene Symbol KIF14
Gene ID (NCBI) 9928
RRIDAB_2880327
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15058
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KIF14 antibody 26000-1-APDownload protocol
IHC protocol for KIF14 antibody 26000-1-APDownload protocol
IP protocol for KIF14 antibody 26000-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols