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- KD/KO Validated
KIF20A Monoclonal antibody
KIF20A Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Rat, Mouse and More (2)
Applications
IF, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2A8E3
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, HEK-293 cells, Jurkat cells, HL-60 cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells |
| Positive IHC detected in | human breast cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67190-1-Ig targets KIF20A in WB, IF, IHC, ELISA applications and shows reactivity with Human, Rat, Mouse samples.
| Tested Applications | IF, IHC, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | Human, Rat, Mouse |
| Cited Reactivity | human, mouse |
| Immunogen | KIF20A fusion protein Ag8916 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | kinesin family member 20A |
| Synonyms | FLJ21151, GG10_2, KIF20A, kinesin family member 20A, Kinesin like protein KIF20A, MKLP2, RAB6KIFL, Rabkinesin 6 |
| Calculated Molecular Weight | 890 aa, 100 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC012999 |
| Gene Symbol | KIF20A |
| Gene ID (NCBI) | 10112 |
| RRID | AB_2882485 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O95235 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KIF20A, also known as RAB6KIFL or MKlp2, is a kinesin originally identified as a binding partner for the rab6 GTPase involved in protein transport at the Golgi apparatus. KIF20A belongs to a large family of motor proteins that accumulate in mitotic cells and is required for normal cell division. KIF20A is thought to be involved in carcinogenesis. In normal tissues KIF20A is almost undetectable while it is overexpressed in various cancers. KIF20A has been identified as a novel promising candidate for anticancer immunotherapy.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KIF20A antibody 67190-1-Ig | Download protocol |
| IHC protocol for KIF20A antibody 67190-1-Ig | Download protocol |
| IF protocol for KIF20A antibody 67190-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Res Proteomic mapping and targeting of mitotic pericentriolar material in tumors bearing centrosome amplification. | ||
Genomics Upregulation of the key biomarker kinesin family member 20A (KIF20A) is associated with the pulmonary artery hypertension | ||
Nat Commun An oocyte meiotic midbody cap is required for developmental competence in mice | ||
Mol Cancer Res Overlaid transcriptome and proteome analyses identify mitotic kinesins as important targets of arylsulfonamide-mediated RBM39 degradation | ||
Mol Cancer Res Overlaid Transcriptional and Proteome Analyses Identify Mitotic Kinesins as Important Targets of Arylsulfonamide-Mediated RBM39 Degradation |
