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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated KLF5 Monoclonal antibody

KLF5 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C11C5

Cat No : CL488-66850

Print datasheet

Synonyms

BTE binding protein 2, BTEB2, CKLF, Colon krueppel like factor, GC box binding protein 2, IKLF, KLF5, Krueppel like factor 5, Transcription factor BTEB2

验证数据展示

  • FC experiment of HeLa using CL488-66850

    FC experiment of HeLa using CL488-66850

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human KLF5 (CL488-66850, Clone:2C11C5) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66850-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66850 targets KLF5 in FC (Intra) applications and shows reactivity with Human, mouse samples.

Tested Applications FC (Intra)
Tested Reactivity Human, mouse
Immunogen KLF5 fusion protein Ag15403 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Kruppel-like factor 5 (intestinal)
Synonyms BTE binding protein 2, BTEB2, CKLF, Colon krueppel like factor, GC box binding protein 2, IKLF, KLF5, Krueppel like factor 5, Transcription factor BTEB2
Calculated Molecular Weight 457 aa, 51 kDa
Observed Molecular Weight 51-57 kDa
GenBank Accession NumberBC042131
Gene Symbol KLF5
Gene ID (NCBI) 688
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13887
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 KLF5 antibody CL488-66850Download protocol
Standard Protocols
Click here to view our Standard Protocols