KLF6 Monoclonal antibody

KLF6 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2F8B3

Cat No : 67297-1-Ig

Print datasheet

Synonyms

B cell derived protein 1, COPEB, CPBP, DKFZp686N0199, GBF, KLF6, Krueppel like factor 6, Kruppel like factor 6, PAC1, Proto oncogene BCD1, ST12, Transcription factor Zf9, ZF9



经过测试的应用

Positive WB detected inJAR cells, NCCIT cells, L02 cells, Jurkat cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

67297-1-Ig targets KLF6 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA
Cited Applications WB
Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse
Immunogen KLF6 fusion protein Ag6859 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Kruppel-like factor 6
Synonyms B cell derived protein 1, COPEB, CPBP, DKFZp686N0199, GBF, KLF6, Krueppel like factor 6, Kruppel like factor 6, PAC1, Proto oncogene BCD1, ST12, Transcription factor Zf9, ZF9
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32-42 kDa
GenBank Accession NumberBC000311
Gene Symbol KLF6
Gene ID (NCBI) 1316
RRIDAB_2882561
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99612
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLF6(krupple-like factor 6) is a zinc finger transcription factor and tumor suppressor with biological activities and transcriptional targets in growing range. It is highly expressed in placenta followed by spleen, thymus, prostate, testis, small intestine and colon. One key target gene of KLF6 in endoglin, which is a homodimeric cell membrane glycoprotein and TGF-β auxiliary receptor, owns a pro-angiogenic role in endothelial cells and is also involved in malignant progression. Also KLF6 induces apoptosis in prostate cancer cells through upregulation of ATF3. KLF6 was normal detected as a 46kDa protein, which is larger than translation product(32kDa), because of post-translational modification including both phosphorylation and glycosylation. This antibody raise against the full length of KLF6 gene of human origin, and can recognize both of KLF6, included KLF6(~32kDa), p-KLF6(~35-46kDa). Otherwise, there may be a non-specific band(50kDa) showed in detection

实验方案

Product Specific Protocols
WB protocol for KLF6 antibody 67297-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

BMC Mol Cell Biol

LncRNA LUCAT1/miR-181a-5p axis promotes proliferation and invasion of breast cancer via targeting KLF6 and KLF15.

Authors - Yun Liu
mouse,humanWB

Cell Signal

Mammalian cleavage factor 25 targets KLF14 to inhibit hepatic stellate cell activation and liver fibrosis

Authors - Xiaoyan Chen