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Cytokeratin 10-specific Polyclonal antibody

Cytokeratin 10-specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16855-1-AP

Print datasheet

Synonyms

KRT10, Cytokeratin-10, Cytokeratin,Keratin, Cytokeratin 10, CK-10

验证数据展示

  • IHC staining of human skin cancer using 16855-1-AP

    IHC staining of human skin cancer using 16855-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT10 antibody included
  • IHC staining of human skin cancer using 16855-1-AP

    IHC staining of human skin cancer using 16855-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT10 antibody included
  • WB analysis using 16855-1-AP

    WB analysis using 16855-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16855-1-AP (Cytokeratin 10-specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse skin tissue, rat skin tissue
Positive IHC detected inhuman skin cancer tissue, human breast cancer tissue, mouse skin tissue, rat skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy KRT10 Ready-To-Use IHC Kit. KRT10 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16855-1-AP targets Cytokeratin 10-specific in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name keratin 10
Synonyms KRT10, Cytokeratin-10, Cytokeratin,Keratin, Cytokeratin 10, CK-10
Calculated Molecular Weight 59 kDa
Observed Molecular Weight59 kDa
GenBank Accession NumberNM_000421
Gene Symbol Cytokeratin 10
Gene ID (NCBI) 3858
RRIDAB_10792092
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13645
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. As a type I keratin, keratin 10 is a suprabasal marker of differentiation in stratified squamous epithelia. This antibody is specifically against KRT10.

实验方案

Product Specific Protocols
WB protocol for Cytokeratin 10-specific antibody 16855-1-APDownload protocol
IHC protocol for Cytokeratin 10-specific antibody 16855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols