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验证数据展示

  • WB analysis of A431 using 10143-1-AP

    WB analysis of A431 using 10143-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 10143-1-AP

    IHC staining of human lung cancer using 10143-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with KRT14 antibody included
  • IHC staining of human lung cancer using 10143-1-AP

    IHC staining of human lung cancer using 10143-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with KRT14 antibody included
  • IF Staining of HaCaT using 10143-1-AP

    IF Staining of HaCaT using 10143-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Cytokeratin 14 antibody (10143-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse olfactory epithelium tissue using 10143-1-AP

    IF Staining of mouse olfactory epithelium tissue using 10143-1-AP

    IF result of CK14 (10143-1-AP, 1:400) with 1% PLP fixed basal quiescent stem cells of the adult mouse olfactory epithelium. (Green: CK14; Blue: DAPI). Basal lamina is marked by an arrow head. By Brian Lin, Tufts University.

  • FC experiment of A431 using 10143-1-AP

    FC experiment of A431 using 10143-1-AP

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 14 (10143-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IP experiment of A431 using 10143-1-AP

    IP experiment of A431 using 10143-1-AP

    IP result of anti-Cytokeratin 14 (IP:10143-1-AP, 3ug; Detection:10143-1-AP 1:1000) with A431 cells lysate 1200ug.

  • IHC staining of human lung squamous cell carcinoma using 10143-1-AP

    IHC staining of human lung squamous cell carcinoma using 10143-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:8000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KRT14 antibody included
  • Featured Product
  • KD/KO验证

Cytokeratin 14 Polyclonal antibody

Cytokeratin 14 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 10143-1-AP

产品说明书

引用文献(101)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

别名

KRT14, CK 14, CK14, CK-14, Cytokeratin,Keratin

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, rat skin tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue, human cervical cancer tissue, human lung squamous cell carcinoma tissue, human oesophagus cancer tissue, human skin tissue, human skin cancer tissue, mouse skin tissue, rat skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHaCaT cells, HeLa cells, HepG2 cells, mouse olfactory epithelium tissue
Positive FC (Intra) detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy KRT14 Ready-To-Use IHC Kit. KRT14 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10143-1-AP targets Cytokeratin 14 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原

CatNo: Ag0188

Product name: Recombinant human KRT14 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 262-472 aa of BC002690

Sequence: GQVGGDVNVEMDAAPGVDLSRILNEMRDQYEKMAEKNRKDAEEWFFTKTEELNREVATNSELVQSGKSEISELRRTMQNLEIELQSQLSMKASLENSLEETKGRYCMQLAQIQEMIGSVEEQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLSSSQFSSGSQSSRDVTSSSRQIRTKVMDVHDGKVVSTHEQVLRTKN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 keratin 14
别名 KRT14, CK 14, CK14, CK-14, Cytokeratin,Keratin
计算分子量 472 aa, 52 kDa
观测分子量 47-50 kDa
GenBank蛋白编号BC002690
基因名称 Cytokeratin 14
Gene ID (NCBI) 3861
RRIDAB_2134831
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP02533
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.

实验方案

Product Specific Protocols
FC protocol for Cytokeratin 14 antibody 10143-1-APDownload protocol
IF protocol for Cytokeratin 14 antibody 10143-1-APDownload protocol
IHC protocol for Cytokeratin 14 antibody 10143-1-APDownload protocol
IP protocol for Cytokeratin 14 antibody 10143-1-APDownload protocol
WB protocol for Cytokeratin 14 antibody 10143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIHC

Cell Stem Cell

Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency.

Authors - Brian Lin
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humanIF

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mouseIF

Adv Sci (Weinh)

Photothermal-Responsive Soluble Microneedle Patches for Meibomian Gland Dysfunction Therapy

Authors - Fei Yu
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mouseIF

Nat Commun

Malfunction of airway basal stem cells plays a crucial role in pathophysiology of tracheobronchopathia osteoplastica.

Authors - Yue Hong
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humanIF

Nat Commun

Human induced pluripotent stem cell-derived vocal fold mucosa mimics development and responses to smoke exposure.

Authors - Vlasta Lungova
View Article
mouseIF

Biomaterials

MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system.

Authors - Tian Wang
View Article
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