验证数据展示

WB analysis of A431 using 10143-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 10143-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).
Ready-to-use IHC kits available with KRT14 antibody included
IHC staining of human lung cancer using 10143-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).
Ready-to-use IHC kits available with KRT14 antibody included
IF Staining of HaCaT using 10143-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using Cytokeratin 14 antibody (10143-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse olfactory epithelium tissue using 10143-1-AP
IF result of CK14 (10143-1-AP, 1:400) with 1% PLP fixed basal quiescent stem cells of the adult mouse olfactory epithelium. (Green: CK14; Blue: DAPI). Basal lamina is marked by an arrow head. By Brian Lin, Tufts University.
FC experiment of A431 using 10143-1-AP
1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Cytokeratin 14 (10143-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IP experiment of A431 using 10143-1-AP
IP result of anti-Cytokeratin 14 (IP:10143-1-AP, 3ug; Detection:10143-1-AP 1:1000) with A431 cells lysate 1200ug.

Featured Product
KD/KO验证

Cytokeratin 14 Polyclonal antibody

Cytokeratin 14 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10143-1-AP

产品说明书

引用文献(98)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

别名

KRT14, CK 14, CK14, CK-14, Cytokeratin,Keratin

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 555 CoraLite® Plus 647 CoraLite®594

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (98)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	A431 cells, rat skin tissue
Positive IP detected in	A431 cells
Positive IHC detected in	human lung cancer tissue, human cervical cancer tissue, human breast cancer tissue, human skin tissue, human skin cancer tissue, human oesophagus cancer tissue, mouse skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HaCaT cells, HeLa cells, HepG2 cells, mouse olfactory epithelium tissue
Positive FC (Intra) detected in	A431 cells

Planning an IHC experiment? We recommend our IHCeasy KRT14 Ready-To-Use IHC Kit. KRT14 primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 1 publications below
WB	See 16 publications below
IHC	See 26 publications below
IF	See 59 publications below

产品信息

10143-1-AP targets Cytokeratin 14 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用	WB, IHC, IF
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat
免疫原	CatNo: Ag0188 Product name: Recombinant human KRT14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 262-472 aa of BC002690 Sequence: GQVGGDVNVEMDAAPGVDLSRILNEMRDQYEKMAEKNRKDAEEWFFTKTEELNREVATNSELVQSGKSEISELRRTMQNLEIELQSQLSMKASLENSLEETKGRYCMQLAQIQEMIGSVEEQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLSSSQFSSGSQSSRDVTSSSRQIRTKVMDVHDGKVVSTHEQVLRTKN 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	keratin 14
别名	KRT14, CK 14, CK14, CK-14, Cytokeratin,Keratin
计算分子量	472 aa, 52 kDa
观测分子量	47-50 kDa
GenBank蛋白编号	BC002690
基因名称	Cytokeratin 14
Gene ID (NCBI)	3861
RRID	AB_2134831
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P02533
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.

实验方案

Product Specific Protocols
IF protocol for Cytokeratin 14 antibody 10143-1-AP	Download protocol
IHC protocol for Cytokeratin 14 antibody 10143-1-AP	Download protocol
IP protocol for Cytokeratin 14 antibody 10143-1-AP	Download protocol
WB protocol for Cytokeratin 14 antibody 10143-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	IHC	Cell Stem Cell Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency. Authors - Brian Lin View Article
mouse	IF	Adv Sci (Weinh) Photothermal-Responsive Soluble Microneedle Patches for Meibomian Gland Dysfunction Therapy Authors - Fei Yu View Article
mouse	IF	Nat Commun Malfunction of airway basal stem cells plays a crucial role in pathophysiology of tracheobronchopathia osteoplastica. Authors - Yue Hong View Article
human	IF	Nat Commun Human induced pluripotent stem cell-derived vocal fold mucosa mimics development and responses to smoke exposure. Authors - Vlasta Lungova View Article
mouse	IF	Biomaterials MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system. Authors - Tian Wang View Article
human	WB	Oncogene EB-virus latent membrane protein 1 potentiates the stemness of nasopharyngeal carcinoma via preferential activation of PI3K/AKT pathway by a positive feedback loop. Authors - C-F Yang View Article

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验证数据展示

WB analysis of A431 using 10143-1-AP

IHC staining of human lung cancer using 10143-1-AP

IHC staining of human lung cancer using 10143-1-AP

IF Staining of HaCaT using 10143-1-AP

IF Staining of mouse olfactory epithelium tissue using 10143-1-AP

FC experiment of A431 using 10143-1-AP

IP experiment of A431 using 10143-1-AP

Cytokeratin 14 Polyclonal antibody

Cytokeratin 14 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Injury Induces Endogenous Reprogramming and Dedifferentiation of Neuronal Progenitors to Multipotency.

Photothermal-Responsive Soluble Microneedle Patches for Meibomian Gland Dysfunction Therapy

Malfunction of airway basal stem cells plays a crucial role in pathophysiology of tracheobronchopathia osteoplastica.

Human induced pluripotent stem cell-derived vocal fold mucosa mimics development and responses to smoke exposure.

MSC-derived exosomes protect against oxidative stress-induced skin injury via adaptive regulation of the NRF2 defense system.

EB-virus latent membrane protein 1 potentiates the stemness of nasopharyngeal carcinoma via preferential activation of PI3K/AKT pathway by a positive feedback loop.