Back

Cytokeratin 14 Polyclonal antibody 33 Publications

Rabbit Polyclonal| Catalog number: 10143-1-AP

Featured Product KD/KO validated

浓度: 360 μg/ml

点击咨询在线客服

Related Primary Antibodies

Applications

Tested Applications:
FC, IF, IHC, IP, WB,ELISA
Application not tested? Please see our Guarantee


Cited Applications:
IF, IHC, WB


Images See all

WB analysis of A431 using 10143-1-AP

A431 cells were subjected to SDS PAGE followed by western blot with 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IHC staining of human lung cancer using 10143-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 10x lens).

IHC staining of human lung cancer using 10143-1-AP

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10143-1-AP (Cytokeratin 14 antibody at dilution of 1:200 (under 40x lens).

IF Staining of HeLa using 10143-1-AP

Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 10143-1-AP (Cytokeratin 14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

IF Staining of mouse olfactory epithelium tissue using 10143-1-AP

IF result of CK14 (10143-1-AP, 1:400) with 1% PLP fixed basal quiescent stem cells of the adult mouse olfactory epithelium. (Green: CK14; Blue: DAPI). Basal lamina is marked by an arrow head. By Brian Lin, Tufts University.

IP experiment of A431 using 10143-1-AP

IP Result of anti-Cytokeratin 14 (IP:10143-1-AP, 3ug; Detection:10143-1-AP 1:1000) with A431 cells lysate 1200ug.

FC experiment of A431 using 10143-1-AP

1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 14 (10143-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Samples were fixed with 90% MeOH.

Species specificity:
human, mouse, rat
Positive WB detected in:
A431 cells, rat skin tissue
Positive IP detected in:
A431 cells
Positive IHC detected in:
human lung cancer tissue, human breast cancer tissue, human cervical cancer tissue, human oesophagus cancer tissue, human skin tissue, human skin cancer tissue, mouse skin tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells, mouse olfactory epithelium tissue
Positive FC detected in:
A431 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:50-1:500

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Cytokeratin 14 fusion protein Ag0188
Full name:
keratin 14
Calculated molecular weight:
472 aa, 52 kDa
Observed molecular weight:
47-50 kDa
GenBank accession number:
BC002690
Gene ID (NCBI):
3861
Gene symbol
KRT14
Synonyms
CK 14, CK14, Cytokeratin 14, EBS3, EBS4, K14, keratin 14, KRT14, NFJ

Background

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 14 is a type I cytokeratin. It is usually found as a heterotetramer with keratin 5. Keratins K14 and K5 have long been considered to be biochemical markers of the stratified squamous epithelia, including epidermis.

Protocols

PRODUCT SPECIFIC PROTOCOLS

STANDARD PROTOCOLS

Click here to view our Standard Protocols

Back
to top