Cytokeratin 14 Monoclonal antibody
Cytokeratin 14 Monoclonal Antibody for IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, pig, rat
Applications
IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2G1E2
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, mouse skin tissue |
Positive IHC detected in | human lung cancer tissue, human cervical cancer tissue, human skin tissue, human breast hyperplasia tissue, human skin cancer tissue, rat skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | A431 cells, mouse skin tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:400-1:800 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
60320-1-Ig targets Cytokeratin 14 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, pig, rat samples.
Tested Applications | IF-P, IHC, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human, mouse, pig, rat |
Cited Reactivity | human, mouse |
Immunogen | Cytokeratin 14 fusion protein Ag17559 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | keratin 14 |
Synonyms | Cell and organelle markers, CK 14, CK14, Cytokeratin 14, Cytoskeleton Marker, EBS3, EBS4, K14, keratin 14, KRT14, NFJ |
Calculated Molecular Weight | 472 aa, 52 kDa |
Observed Molecular Weight | 52 kDa |
GenBank Accession Number | BC002690 |
Gene Symbol | KRT14 |
Gene ID (NCBI) | 3861 |
RRID | AB_2881431 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | P02533 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Cytokeratin 14 antibody 60320-1-Ig | Download protocol |
IHC protocol for Cytokeratin 14 antibody 60320-1-Ig | Download protocol |
IF protocol for Cytokeratin 14 antibody 60320-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Small Eco-Friendly and Scalable Synthesis of Fullerenols with High Free Radical Scavenging Ability for Skin Radioprotection. | ||
Breast Cancer Res Anillin regulates breast cancer cell migration, growth, and metastasis by non-canonical mechanisms involving control of cell stemness and differentiation. | ||
Environ Sci Technol Graphene Quantum Dots Disrupt Embryonic Stem Cell Differentiation by Interfering with the Methylation Level of Sox2. | ||
J Nanobiotechnology Exosomes from human induced pluripotent stem cells-derived keratinocytes accelerate burn wound healing through miR-762 mediated promotion of keratinocytes and endothelial cells migration. | ||
Stem Cell Res Ther Bone marrow mesenchymal stem cells facilitate diabetic wound healing through the restoration of epidermal cell autophagy via the HIF-1α/TGF-β1/SMAD pathway |