CoraLite® Plus 488-conjugated CD107a / LAMP1 Polyclonal antibody
CD107a / LAMP1 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
验证数据展示
经过测试的应用
Positive FC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy LAMP1/CD107a Ready-To-Use IHC Kit. LAMP1/CD107a primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 21997-1-AP
推荐稀释比
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of CL488-21997 is CD107a / LAMP1 Fusion Protein expressed in E. coli.
Tested Applications | FC (Intra) |
Tested Reactivity | human |
Immunogen | CD107a / LAMP1 fusion protein Ag17367 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | lysosomal-associated membrane protein 1 |
Synonyms | CD107a, Cell and organelle markers, LAMP 1, LAMP1, LAMPA, LGP120, lysosomal marker, Lysosome Marker |
Calculated Molecular Weight | 417 aa, 45 kDa |
Observed Molecular Weight | 120 kDa |
GenBank Accession Number | BC021288 |
Gene Symbol | LAMP1 |
Gene ID (NCBI) | 3916 |
ENSEMBL Gene ID | ENSG00000185896 |
RRID | AB_2919188 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P11279 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
LAMP1 (also known as CD107a) is a 90-120 kDa heavily glycosylated membrane protein enriched in the lysosomal membrane. LAMP1 functions to provide selectins with carbohydrate ligands. This protein has also been shown to be a marker of degranulation on lymphocytes such as CD8+ and NK cells and may also play a role in tumor cell differentiation and metastasis.
实验方案
Product Specific Protocols | |
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FC protocol for CL Plus 488 CD107a / LAMP1 antibody CL488-21997 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |