CD107b / LAMP2 Polyclonal antibody

CD107b / LAMP2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Pig and More (2)

Applications

IHC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 27823-1-AP

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Synonyms

CD107b, Cell and organelle markers, LAMP 2, LAMP2, LAMPB, LGP110, Lysosome Marker



经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, pig liver tissue, human placenta tissue
Positive IHC detected inhuman liver tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy LAMP2 Ready-To-Use IHC Kit. LAMP2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27823-1-AP targets CD107b / LAMP2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Pig samples.

Tested Applications IHC, WB, ELISA
Cited Applications IF, IHC, WB
Tested Reactivity Human, Pig
Cited Reactivityhuman, pig
Immunogen CD107b / LAMP2 fusion protein Ag27183 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name lysosomal-associated membrane protein 2
Synonyms CD107b, Cell and organelle markers, LAMP 2, LAMP2, LAMPB, LGP110, Lysosome Marker
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 120 kDa
GenBank Accession NumberBC002965
Gene Symbol LAMP2
Gene ID (NCBI) 3920
ENSEMBL Gene IDENSG00000005893
RRIDAB_2880983
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13473
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may plays a role in tumor cell metastasis. It may also functions in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N linked and O linked oligosaccharides.

实验方案

Product Specific Protocols
WB protocol for CD107b / LAMP2 antibody 27823-1-APDownload protocol
IHC protocol for CD107b / LAMP2 antibody 27823-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Extracell Vesicles

Extracellular vesicle-mediated delivery of circDYM alleviates CUS-induced depressive-like behaviours.

Authors - Xiaoyu Yu
WB

Autophagy

USP19 (ubiquitin specific peptidase 19) promotes TBK1 (TANK-binding kinase 1) degradation via chaperone-mediated autophagy.

Authors - Xibao Zhao
pigIF

Autophagy

Extracellular vesicles originating from autophagy mediate an antibody-resistant spread of classical swine fever virus in cell culture

Authors - Tao Wang
humanWB

Oxid Med Cell Longev

Restoration of Autophagic Flux Rescues Oxidative Damage and Mitochondrial Dysfunction to Protect against Intervertebral Disc Degeneration.

Authors - Liang Kang
pigWB

J Anim Sci Biotechnol

Glyphosate exposure deteriorates oocyte meiotic maturation via induction of organelle dysfunctions in pigs.

Authors - Chunhua Xing
IF

J Neurosci

Effect of Aβ oligomers on neuronal APP triggers a vicious cycle leading to the propagation of synaptic plasticity alterations to healthy neurons.

Authors - Marta Rolland