- Featured Product
- KD/KO Validated
LC3 Recombinant antibody
LC3 Recombinant Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat, Pig and More (1)
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
5P12
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, Chloroquine treated HEK-293 cells, Chloroquine treated HeLa cells, pig brain tissue, rat brain tissue, mouse brain tissue |
Positive IHC detected in | human colon cancer tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Chloroquine treated HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:5000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF) | IF : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
IF | See 1 publications below |
产品信息
81004-1-RR targets LC3 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
Tested Applications | IF/ICC, IHC, WB, ELISA |
Cited Applications | WB, IF |
Tested Reactivity | Human, Mouse, Rat, Pig |
Cited Reactivity | mouse |
Immunogen | LC3 fusion protein Ag6144 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | microtubule-associated protein 1 light chain 3 beta |
Synonyms | LC3, LC3B, MAP1A/1BLC3, MAP1A/MAP1B LC3 B, MAP1A/MAP1B light chain 3 B, MAP1ALC3 |
Calculated Molecular Weight | 15 kDa |
Observed Molecular Weight | 14-18 kDa |
GenBank Accession Number | BC067797 |
Gene Symbol | MAP1LC3B |
Gene ID (NCBI) | 81631 |
ENSEMBL Gene ID | ENSG00000140941 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q9GZQ8 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for LC3 antibody 81004-1-RR | Download protocol |
IHC protocol for LC3 antibody 81004-1-RR | Download protocol |
IF protocol for LC3 antibody 81004-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Int J Mol Sci Combination of Kaempferol and Docetaxel Induces Autophagy in Prostate Cancer Cells In Vitro and In Vivo |