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LRRC8C Polyclonal antibody

LRRC8C Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 21601-1-AP

Print datasheet

Synonyms

AD158, FAD158, LRRC8C

验证数据展示

  • WB analysis using 21601-1-AP

    WB analysis using 21601-1-AP

    THP-1 cells were subjected to SDS PAGE followed by western blot with 21601-1-AP (LRRC8C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 21601-1-AP

    IHC staining of mouse heart using 21601-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21601-1-AP (LRRC8C antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 21601-1-AP

    IHC staining of mouse heart using 21601-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21601-1-AP (LRRC8C antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 21601-1-AP

    IF Staining of HepG2 using 21601-1-AP

    Immunofluorescent analysis of HepG2 cells, using LRRC8C antibody 21601-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inTHP-1 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21601-1-AP is LRRC8C Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen LRRC8C fusion protein Ag16213 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name leucine rich repeat containing 8 family, member C
Synonyms AD158, FAD158, LRRC8C
Calculated Molecular Weight 803 aa, 92 kDa
Observed Molecular Weight92 kDa
GenBank Accession NumberBC113973
Gene Symbol LRRC8C
Gene ID (NCBI) 84230
RRIDAB_10733648
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TDW0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for LRRC8C antibody 21601-1-APDownload protocol
IHC protocol for LRRC8C antibody 21601-1-APDownload protocol
IF protocol for LRRC8C antibody 21601-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Biomedicines

Astrocytes-Derived Small Extracellular Vesicles Hinder Glioma Growth

Authors - Carmela Serpe
View Article