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LYN Polyclonal antibody

LYN Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 18135-1-AP

Print datasheet

Synonyms

JTK8, LYN, Tyrosine protein kinase Lyn

验证数据展示

  • WB analysis of HeLa using 18135-1-AP

    WB analysis of HeLa using 18135-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18135-1-AP (LYN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 18135-1-AP

    IP experiment of HeLa using 18135-1-AP

    IP result of anti-LYN (IP:18135-1-AP, 4ug; Detection:18135-1-AP 1:1000) with HeLa cells lysate 1200ug.

  • IHC staining of human kidney using 18135-1-AP

    IHC staining of human kidney using 18135-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18135-1-AP (LYN Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with LYN antibody included
  • IHC staining of human kidney using 18135-1-AP

    IHC staining of human kidney using 18135-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18135-1-AP (LYN Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with LYN antibody included
  • IF Staining of HeLa using 18135-1-AP

    IF Staining of HeLa using 18135-1-AP

    Immunofluorescent analysis of HeLa cells using 18135-1-AP (LYN antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of Jurkat using 18135-1-AP

    FC experiment of Jurkat using 18135-1-AP

    1X10^6 Jurkat cells were stained with 0.2ug LYN antibody (18135-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

经过测试的应用

Positive WB detected inHeLa cells, HL-60 cells, Jurkat cells, K-562 cells, L02 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman kidney tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy LYN Ready-To-Use IHC Kit. LYN primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IHCSee 1 publications below

产品信息

The immunogen of 18135-1-AP is LYN Fusion Protein expressed in E. coli.

Tested Applications FC, IF/ICC, IHC, IP, WB,ELISA
Cited ApplicationsIHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen LYN fusion protein Ag12723 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name v-yes-1 Yamaguchi sarcoma viral related oncogene homolog
Synonyms JTK8, LYN, Tyrosine protein kinase Lyn
Calculated Molecular Weight 512 aa, 59 kDa
Observed Molecular Weight 53-56 kDa, 66-70 kDa
GenBank Accession NumberBC075002
Gene Symbol LYN
Gene ID (NCBI) 4067
RRIDAB_11042319
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP07948
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LYN, also named as JTK8, p53Lyn and p56Lyn, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It is a non-receptor tyrosine-protein kinase that transmits signals from cell surface receptors and plays an important role in the regulation of innate and adaptive immune responses, hematopoiesis, responses to growth factors and cytokines, integrin signaling, but also responses to DNA damage and genotoxic agents. LYN is a component of the mTOR (mammalian target of rapamycin) pathway.

实验方案

Product Specific Protocols
WB protocol for LYN antibody 18135-1-APDownload protocol
IHC protocol for LYN antibody 18135-1-APDownload protocol
IF protocol for LYN antibody 18135-1-APDownload protocol
IP protocol for LYN antibody 18135-1-APDownload protocol
FC protocol for LYN antibody 18135-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Cell Dev Biol

Immune Characteristics of LYN in Tumor Microenvironment of Gliomas.

Authors - Chonghua Jiang
View Article