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  • Featured Product
  • KD/KO Validated

Lamin A/C Recombinant antibody, PBS Only

Lamin A/C Recombinant Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

5I16

Cat No : 81042-1-PBS

Print datasheet

Synonyms

70 kDa lamin, CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, HGPS, IDC, lamin A, lamin A/C, LDP1, LFP, LGMD1B, LMN1, LMNA, LMNC, Prelamin A/C, PRO1

验证数据展示

  • WB analysis using 81042-1-RR (same clone as 81042-1-PBS)

    WB analysis using 81042-1-RR (same clone as 81042-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81042-1-RR (Lamin A/C antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 81042-1-RR (same clone as 81042-1-PBS)

    WB analysis of HeLa using 81042-1-RR (same clone as 81042-1-PBS)

    WB result of Lamin A/C antibody (81042-1-RR; 1:120000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin A/C transfected HeLa cells. This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 81042-1-RR (same clone as 81042-1-PBS)

    IHC staining of mouse heart using 81042-1-RR (same clone as 81042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81042-1-RR (Lamin A/C antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Lamin A/C antibody included
  • IHC staining of mouse heart using 81042-1-RR (same clone as 81042-1-PBS)

    IHC staining of mouse heart using 81042-1-RR (same clone as 81042-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 81042-1-RR (Lamin A/C antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Lamin A/C antibody included
  • IF Staining of HepG2 using 81042-1-RR (same clone as 81042-1-PBS)

    IF Staining of HepG2 using 81042-1-RR (same clone as 81042-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Lamin A/C antibody (81042-1-RR, Clone: 5I16 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81042-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Lamin A/C Ready-To-Use IHC Kit. Lamin A/C primary antibody included.

产品信息

81042-1-PBS targets Lamin A/C in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF
Tested Reactivity Human, mouse, rat
Immunogen Lamin A/C fusion protein Ag0408 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name lamin A/C
Synonyms 70 kDa lamin, CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, HGPS, IDC, lamin A, lamin A/C, LDP1, LFP, LGMD1B, LMN1, LMNA, LMNC, Prelamin A/C, PRO1
Calculated Molecular Weight 65 kDa
Observed Molecular Weight65-70 kDa
GenBank Accession NumberBC003162
Gene Symbol LMNA
Gene ID (NCBI) 4000
ENSEMBL Gene IDENSG00000160789
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02545
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Lamin A/C is also named as LMNA, or LMN1. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. The lack of lamin A/C can be as a novel marker for undifferentiated embryonic stem cells and lamin A/C expression is as an early indicator of differentiation (PMID: 16179429). Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. This protein has 4 isoforms produced by alternative splicing with the molecular weight of 74 kDa, 65 kDa, 70 kDa and 64 kDa. This antibody can recognize 4 isoforms of Lamin A/C.