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- KD/KO Validated
MAD2L1 Polyclonal antibody
MAD2L1 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Daudi cells, rat liver tissue, HEK-293 cells, HepG2 cells, Raji cells, THP-1 cells, HeLa cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human liver cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy MAD2L1 Ready-To-Use IHC Kit. MAD2L1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10337-1-AP targets MAD2L1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | IF, IHC, WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | MAD2L1 fusion protein Ag0307 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | MAD2 mitotic arrest deficient-like 1 (yeast) |
| Synonyms | HSMAD2, MAD2, MAD2 like protein 1, MAD2L1 |
| Calculated Molecular Weight | 24 kDa |
| Observed Molecular Weight | 25-30 kDa |
| GenBank Accession Number | BC000356 |
| Gene Symbol | MAD2L1 |
| Gene ID (NCBI) | 4085 |
| RRID | AB_2139365 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13257 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MAD2 mitotic arrest deficient-like 1 (yeast) (MAD2L1, synonyms: MAD2, HSMAD2) is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is localized to human chromosome band 5q23.3 by in situ hybridization.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MAD2L1 antibody 10337-1-AP | Download protocol |
| IHC protocol for MAD2L1 antibody 10337-1-AP | Download protocol |
| IF protocol for MAD2L1 antibody 10337-1-AP | Download protocol |
| IP protocol for MAD2L1 antibody 10337-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv The cohesin release factor Wapl interacts with Bub3 to govern SAC activity in female meiosis I. | ||
J Cell Biol Loss of centromeric RNA activates the spindle assembly checkpoint in mammalian female meiosis I. | ||
Oncogene KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells. | ||
Cancer Res Deficiency of pRb family proteins and p53 in invasive urothelial tumorigenesis. | ||
