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验证数据展示

  • WB analysis using 29167-1-AP

    WB analysis using 29167-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29167-1-AP (TAK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 29167-1-AP

    IHC staining of human prostate cancer using 29167-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 29167-1-AP (TAK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MAP3K7/TAK1 antibody included

TAK1 Polyclonal antibody

TAK1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29167-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, ELISA

别名

MAP3K7, TAK1, TGF beta activated kinase 1, TGF1a

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MAP3K7/TAK1 Ready-To-Use IHC Kit. MAP3K7/TAK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29167-1-AP targets TAK1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原 TAK1 fusion protein Ag30725 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 mitogen-activated protein kinase kinase kinase 7
别名 MAP3K7, TAK1, TGF beta activated kinase 1, TGF1a
计算分子量 579 aa, 64 kDa
观测分子量64-70 kDa
GenBank蛋白编号BC017715
基因名称 TAK1
Gene ID (NCBI) 6885
RRIDAB_2918242
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO43318
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

MAP3K7(Mitogen-activated protein kinase kinase kinase 7) is also named as TAK1 and belongs to the MAP kinase kinase kinase subfamily. It plays an important role in the cascades of cellular responses evoked by changes in the environment. It has been linked to interleukin-1 receptor and tumor necrosis factor receptor signaling (PMID: 16186825). It has 4 isoforms (53-55 kDa, 64-70 kDa and 75-80 kDa) produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for TAK1 antibody 29167-1-APDownload protocol
IHC protocol for TAK1 antibody 29167-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols