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MEF2C Polyclonal antibody 28 Publications

Rabbit Polyclonal| Catalog number: 10056-1-AP

Featured Product KD/KO validated

浓度: 470 μg/ml

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Applications

Tested Applications:
IF, IHC, IP, WB, ELISA
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Cited Applications:
chIP, IF, IHC, WB


Images See all

WB analysis of SH-SY5Y using 10056-1-AP

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 10056-1-AP (MEF2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

IP experiment of SH-SY5Y using 10056-1-AP

IP Result of anti-MEF2C (IP:10056-1-AP, 3ug; Detection:10056-1-AP 1:500) with SH-SY5Y cells lysate 2000ug.

IHC staining of human lymphoma using 10056-1-AP

Immunohistochemical analysis of paraffin-embedded human lymphoma using 10056-1-AP (MEF2C antibody) at dilution of 1:50 (under 40x lens).

IF Staining of HepG2 using 10056-1-AP

Immunofluorescent analysis of HepG2 cells using 10056-1-AP (MEF2C antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Species specificity:
human, mouse, rat
Positive WB detected in:
SH-SY5Y cells, mouse colon tissue
Positive IP detected in:
SH-SY5Y cells
Positive IHC detected in:
human lymphoma tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells
Recommended dilution:
WB : 1:500-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
MEF2C fusion protein Ag0020
Full name:
myocyte enhancer factor 2C
Calculated molecular weight:
51 kDa
Observed molecular weight:
52 kDa, 60 kDa
GenBank accession number:
BC156603
Gene ID (NCBI):
4208
Gene symbol
MEF2C
Synonyms
MEF2C, myocyte enhancer factor 2C

Background

MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development[PMID: 20221419]. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission[PMID:18599438]. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis[PMID: 21666133]. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. MEF2C exists some isoforms with MV 50-52 kDa, 47 kDa, and 45 kDa, but modified MEF2C is about 55-60 kDa.

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