验证数据展示
经过测试的应用
| Positive WB detected in | C2C12 cell, HeLa cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14353-1-AP targets MEF2D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
| Cited Applications | WB, IHC, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Immunogen | MEF2D fusion protein Ag5700 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | myocyte enhancer factor 2D |
| Synonyms | Myocyte-specific enhancer factor 2D |
| Calculated Molecular Weight | 56 kDa |
| Observed Molecular Weight | 55-60 kDa |
| GenBank Accession Number | BC040949 |
| Gene Symbol | MEF2D |
| Gene ID (NCBI) | 4209 |
| RRID | AB_2878046 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14814 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MEF2D, also named as Myocyte-specific enhancer factor 2D, is a 521 amino acid protein, which belongs to the MEF2 family. MEF2D is expressed in t in myotubes and also in undifferentiated myoblasts. MEF2D as a transcriptional activator which binds specifically to the MEF2 element, found in numerous muscle-specific and stress-induced genes. MEF2D mediates cellular functions not only in skeletal and cardiac muscle development, but also in neuronal differentiation and survival. The calculated molecular weight of MEF2D is 55 kDa, but Phosphorylated MEF2D is 60 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MEF2D antibody 14353-1-AP | Download protocol |
| IHC protocol for MEF2D antibody 14353-1-AP | Download protocol |
| IF protocol for MEF2D antibody 14353-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Alternatively spliced exon regulates context-dependent MEF2D higher-order assembly during myogenesis | ||
Autophagy MEF2D-NR4A1-FAM134B2-mediated reticulophagy contributes to amino acid homeostasis | ||
Ecotoxicol Environ Saf GPX4 degradation via chaperone-mediated autophagy contributes to antimony-triggered neuronal ferroptosis. | ||
J Transl Med Extracellular vesicle miR-32 derived from macrophage promotes arterial calcification in mice with type 2 diabetes via inhibiting VSMC autophagy. | ||
