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MRPS26 Polyclonal antibody

MRPS26 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 15989-1-AP

Print datasheet

Synonyms

C20orf193, GI008, MRP S13, MRP S26, MRPS13, MRPS26, NY BR 87, RPMS13, S13mt, S26mt

验证数据展示

  • IP experiment of HepG2 using 15989-1-AP

    IP experiment of HepG2 using 15989-1-AP

    IP result of anti-MRPS26 (IP:15989-1-AP, 4ug; Detection:15989-1-AP 1:500) with HepG2 cells lysate 3300 ug.

  • WB analysis of HepG2 using 15989-1-AP

    WB analysis of HepG2 using 15989-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 15989-1-AP (MRPS26 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 15989-1-AP

    IHC staining of human liver cancer using 15989-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15989-1-AP (MRPS26 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human skin cancer using 15989-1-AP

    IHC staining of human skin cancer using 15989-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 15989-1-AP (MRPS26 Antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, mouse brain tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman skin cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

15989-1-AP targets MRPS26 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen MRPS26 fusion protein Ag8797 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name mitochondrial ribosomal protein S26
Synonyms C20orf193, GI008, MRP S13, MRP S26, MRPS13, MRPS26, NY BR 87, RPMS13, S13mt, S26mt
Calculated Molecular Weight 205 aa, 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC013018
Gene Symbol MRPS26
Gene ID (NCBI) 64949
RRIDAB_10858479
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYN8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MRPS26 antibody 15989-1-APDownload protocol
IHC protocol for MRPS26 antibody 15989-1-APDownload protocol
IP protocol for MRPS26 antibody 15989-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Life Sci Alliance

Mitochondrial stress response triggered by defects in protein synthesis quality control.

Authors - Uwe Richter
View Article
humanWB

Clin Exp Immunol

Cultured lymphocytes' mitochondrial genome integrity is not altered by cladribine

Authors - Elina Järvinen
View Article