- Featured Product
- KD/KO Validated
B-Myb Polyclonal antibody
B-Myb Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
IHC, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy MYBL2 Ready-To-Use IHC Kit. MYBL2 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18896-1-AP targets B-Myb in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human samples.
| Tested Applications | IHC, IP, WB, ELISA |
| Cited Applications | WB, IP, IF, IHC, ChIP |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Immunogen | B-Myb fusion protein Ag13525 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | v-myb myeloblastosis viral oncogene homolog (avian)-like 2 |
| Synonyms | B MYB, BMYB, B-Myb, Myb like protein 2, Myb related protein B, MYBL2 |
| Calculated Molecular Weight | 79 kDa |
| Observed Molecular Weight | 90 kDa |
| GenBank Accession Number | BC007585 |
| Gene Symbol | MYBL2 |
| Gene ID (NCBI) | 4605 |
| RRID | AB_10644283 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P10244 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MYBL2, also named as BMYB, is a 700 amino acid protein, which is phosphorylated by cyclin A/CDK2 during S-phase. MYBL2 is a transcription factor involved in the regulation of cell survival, proliferation, and differentiation. The calculated molecular weight of MYBL2 is 79 kDa, but modified MYBL2 is about 90 kDa (PMID: 11418655).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for B-Myb antibody 18896-1-AP | Download protocol |
| IHC protocol for B-Myb antibody 18896-1-AP | Download protocol |
| IP protocol for B-Myb antibody 18896-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Oxid Med Cell Longev LncRNA-MALAT1 Regulates Cancer Glucose Metabolism in Prostate Cancer via MYBL2/mTOR Axis. | ||
J Virol Minute Virus of Mice (MVM) inhibits transcription of the cyclin B1 gene during infection. | ||
Cancer Gene Ther A targetable MYBL2-ATAD2 axis governs cell proliferation in ovarian cancer
| ||
Mol Cells Sertad1 Induces Neurological Injury after Ischemic Stroke via the CDK4/p-Rb Pathway. | ||
Mediators Inflamm Knockdown MTDH Inhibits Glioma Proliferation and Migration and Promotes Apoptosis by Downregulating MYBL2 | ||
Cancer Biol Ther The differences of regulatory networks between papillary and anaplastic thyroid carcinoma: an integrative transcriptomics study. |
