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CoraLite®594-conjugated MYH10 Monoclonal antibody

MYH10 Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF-P

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2H8G7

Cat No : CL594-67243

Print datasheet

Synonyms

MYH10, Myosin 10, Myosin heavy chain 10

验证数据展示

  • IF Staining of mouse cerebellum using CL594-67243

    IF Staining of mouse cerebellum using CL594-67243

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CoraLite®594-conjugated MYH10 antibody (CL594-67243, Clone: 2H8G7 ) at dilution of 1:100.

  • IF Staining of mouse cerebellum using CL594-67243

    IF Staining of mouse cerebellum using CL594-67243

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CoraLite®594-conjugated MYH10 antibody (CL594-67243, Clone: 2H8G7 ) at dilution of 1:100.

经过测试的应用

Positive IF detected inmouse cerebellum tissue
Planning an IHC experiment? We recommend our IHCeasy MYH10 Ready-To-Use IHC Kit. MYH10 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67243-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67243 targets MYH10 in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF-P
Tested Reactivity Human, mouse, rat
Immunogen MYH10 fusion protein Ag17069 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name myosin, heavy chain 10, non-muscle
Synonyms MYH10, Myosin 10, Myosin heavy chain 10
Calculated Molecular Weight 1985 aa, 230 kDa
Observed Molecular Weight 200-230 kDa
GenBank Accession NumberBC150634
Gene Symbol MYH10
Gene ID (NCBI) 4628
RRIDAB_2920102
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP35580
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

MYH10 (non-muscle II-b, NM IIB) is a member of non-muscle myosin II which plays fundamental roles in the maintenance of cell morphology, cell adhesion, and migration, as well as cell division. MYH10 is mainly present in nerve cells, megakaryocytes, and other non-muscle cells. It has been reported to mediate centrosome reorientation during cell migration and contribute to ciliogenesis. Overexpression of MYH10 has been observed in breast cancer.

实验方案

Product Specific Protocols
IF protocol for CL594 MYH10 antibody CL594-67243Download protocol
Standard Protocols
Click here to view our Standard Protocols