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  • Featured Product
  • KD/KO Validated

MYO1A Polyclonal antibody

MYO1A Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 17499-1-AP

Print datasheet

Synonyms

BBM I, BBMI, Brush border myosin I, DFNA48, MIHC, MYHL, MYO1A, Myosin I heavy chain, myosin IA

验证数据展示

  • WB analysis of HepG2 using 17499-1-AP

    WB analysis of HepG2 using 17499-1-AP

    WB result of MYO1A antibody (17499-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYO1A transfected HepG2 cells.

  • WB analysis of mouse brain using 17499-1-AP

    WB analysis of mouse brain using 17499-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17499-1-AP (MYO1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 17499-1-AP

    IHC staining of human small intestine using 17499-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17499-1-AP (MYO1A Antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with MYO1A antibody included
  • IHC staining of human small intestine using 17499-1-AP

    IHC staining of human small intestine using 17499-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 17499-1-AP (MYO1A Antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with MYO1A antibody included
  • IF Staining of HepG2 using 17499-1-AP

    IF Staining of HepG2 using 17499-1-AP

    Immunofluorescent analysis of HepG2 cells, using MYO1A antibody 17499-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of HeLa using 17499-1-AP

    IP experiment of HeLa using 17499-1-AP

    IP result of anti-MYO1A (IP:17499-1-AP, 4ug; Detection:17499-1-AP 1:500) with HeLa cells lysate 3200ug.

经过测试的应用

Positive WB detected inmouse brain tissue, HepG2 cells, HeLa cells, A431 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman small intestine tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, Hela cells
Planning an IHC experiment? We recommend our IHCeasy MYO1A Ready-To-Use IHC Kit. MYO1A primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17499-1-AP targets MYO1A in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB,ELISA
Tested Reactivity human, mouse, rat
Immunogen MYO1A fusion protein Ag11682 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name myosin IA
Synonyms BBM I, BBMI, Brush border myosin I, DFNA48, MIHC, MYHL, MYO1A, Myosin I heavy chain, myosin IA
Calculated Molecular Weight 1043 aa, 118 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC059387
Gene Symbol MYO1A
Gene ID (NCBI) 4640
RRIDAB_2148172
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UBC5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for MYO1A antibody 17499-1-APDownload protocol
IHC protocol for MYO1A antibody 17499-1-APDownload protocol
IF protocol for MYO1A antibody 17499-1-APDownload protocol
IP protocol for MYO1A antibody 17499-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols