Mouse CCL3 ELISA Kit0 Publications

Catalog number: KE10023

规格

单价

96 T

¥2600

Images See all

Overview


Product name:
Mouse CCL3 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:
Sandwich

Sensitivity:
0.03 pg/mL

Range:
1.95 - 125 pg/mL

Reacted Species:
Mouse

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Mouse serum 107% 75%-126%
Cell culture supernatants 104% 87%-123%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 86.8 3.8 4.4
2 20 25.8 0.9 3.5
3 20 5.3 0.1 2.5

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 83.8 3.9 4.7
2 24 25.7 0.8 3.0
3 24 5.1 0.3 5.4

Product overview:
KE10023 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse CCL3 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse CCL3. The assay recognizes mouse CCL3. A polyclonal antibody specific for mouse CCL3 has been pre-coated onto the microwells. The mouse CCL3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for mouse CCL3 is added to detect the captured mouse CCL3 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
AI323804, Ccl3, G0S19 1, LD78alpha, LD78-alpha, MIP 1alpha, MIP1 (a), MIP1 alpha, Mip1a, MIP-1-alpha, Scya3, SIS-beta
Background

Chemokine (C-C motif) ligand 3 (CCL3), also known as MIP-1 alpha, belongs to the family of chemokines. CCL3 has been found in the central nervous system and its cognate receptors, CCR1 and CCR5, have been reported to be expressed by astrocytes, microglia and neurons. CCL3 and its receptors, CCR1 and CCR5, also contribute to the development of bone disease in multiple myeloma by supporting tumor growth and regulating osteoclast differentiation. CCL3 is also associated with the regulation of cell growth, angiogenesis, and metastasis of different tumors such as melanoma, renal cell carcinoma, and colorectal cancer. Moreover, CCL3 enhances cell migration and metastasis by up-regulating matrix metalloproteinase-2 (MMP)-2 expression in chondrosarcoma cells.


Back
to top