Mouse IL12 p70 ELISA Kit0 Publications

Catalog number: KE10014

规格

单价

96 T

¥2600

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Overview


Product name:
Mouse IL12 p70 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:
Sandwich

Sensitivity:
2.2 pg/mL

Range:
15.6-1000 pg/mL

Reacted Species:
Mouse

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Serum 101% 84%-115%
Cell culture supernatant 87% 78%-105%

IntraAssay:
SamplenmeanSDCV%
1 20 821.4 55.5 6.8
2 20 191.8 6.4 3.4
3 20 19.7 1.1 5.4

InterAssay:
SamplenmeanSDCV%
1 24 776.8 52.9 6.8
2 24 191.5 12 6.2
3 24 18.7 1.1 6

Product overview:
K10014 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL12 p70 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL12 p70. The assay recognizes mouse IL12 p70. A monoclonal antibody specific for mouse IL12 p70 has been pre-coated onto the microwells. The mouse IL12 p70 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for mouse IL12 p70 is added to detect the captured mouse IL12 p70 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
Il 12a, IL 12p35, Il12a, interleukin 12a, Ll12a, p35
Background

Interleukin-12 (IL12), is a cytokine that is secreted by activated phagocytes and dendritic cells and that induces interferon-γproduction by natural-killer and T lymphocytes. IL12 (referred to as 'p70') is a 70 kDa heterodimer composed of a 35 kDa subunit (IL12a p35) and a 40 kDa subunit (IL12b p40). IL12 is involved in the differentiation of native T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. IL12 plays an important role in the activities of natural killer cells and T lymphocytes. IL12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels. IL12 has been shown to play a critical role in the pathogenesis of a variety of immune-related diseases.


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