K10014 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The mouse IL12 p70 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL12 p70. The assay recognizes mouse IL12 p70. A monoclonal antibody specific for mouse IL12 p70 has been pre-coated onto the microwells. The mouse IL12 p70 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for mouse IL12 p70 is added to detect the captured mouse IL12 p70 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.