Mouse IL27 ELISA Kit0 Publications

Catalog number: KE10016

规格

单价

96 T

¥2600

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Overview


Product name:
Mouse IL27 ELISA Kit

Tests:
1 X 96 well plate

Sample type:
Serum, Plasma, Cell culture supernatant

Assay type:
Sandwich

Sensitivity:
2 pg/mL

Range:
31.25 - 2000 pg/mL

Reacted Species:
Mouse

Tested applications:
Sandwich ELISA

Recovery:
Sample TypeAverageRange
Serum 74% 70%-80%
Cell culture supernatant 97% 87%-109%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 440.7 21.4 4.9
2 20 622.6 35.7 5.7
3 20 1271.1 84.5 6.6

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 346.9 31.6 9.1
2 24 766 56.6 7.4
3 24 1491.3 140.3 9.4

Product overview:
KE10016 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse IL27 ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL27. The assay recognizes mouse IL27. A monoclonal antibody specific for mouse IL27 has been pre-coated onto the microwells. The mouse IL27 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody of biotinylated specific for mouse IL27 is added to detect the captured mouse IL27 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Properties


Storage Instructions:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

Synonyms:
IL 27, IL 27p28, Il27, Il30, interleukin 27, p28
Background

IL27, a member of IL12/IL23 heterodimeric family of cytokines, has pleiotropic properties that can enhance or limit immune responses. IL27 is a heterodimeric cytokine composed of two subunits: IL27a and IL27b. IL27 acts on various cell types, including T cells, B cells, macrophages, dendritic cells, natural killer (NK) cells and non-hematopoietic cells. IL27 plays a critical role in the early regulation of T helper type 1 initiation, and enhances proliferation of naive CD4+T cells and naive B cells. It, however, also exerts anti-inflammatory functions by inhibiting the development of Th17 cells and inducing IL10 producing type 1 regulatory T cells.


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