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WB result of mouse IgG1 isotype control monoclonal antibody (66360-1-Ig, 1:1000, 5 min exposure) in various cell lines. Notice that it does not react with any endogenous protein with MW between 30-250 kDa incubated at room temperature for 1.5 hours
This antibody is used as the isotype control of mouse IgG1. The immunigen of this antibody is Keyhole Limpet Hemocyanin (KLH).