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MultiProTM 5CFLX Anti-Human ATP1A1 (Polyclonal)

ATP1A1 Polyclonal Antibody for Single Cell (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

Single Cell (Intra)

Conjugate

5CFLX

Barcode Sequence

GAACATAATGATCCT

Cat No : G14418-1-5C

Print datasheet

Synonyms

ATP1A1, Na(+)/K(+) ATPase 1, Na(+)/K(+) ATPase alpha 1 subunit, Na+/K+ ATPase 1, Sodium potassium ATPase alpha 1 polypeptide, Sodium pump subunit alpha 1

验证数据展示

  • Single Cell Sequencing experiment G14418-1-5C on Resting PBMC

    Single Cell Sequencing experiment G14418-1-5C on Resting PBMC

    G14418-1-5C was used to stain Resting PBMC and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

  • Single Cell Sequencing experiment G14418-1-5C on PBMC treated with 4hr LPS + TI

    Single Cell Sequencing experiment G14418-1-5C on PBMC treated with 4hr LPS + TI

    G14418-1-5C was used to stain PBMC under 4hr LPS + TI treatment and analyzed with 10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing kit with Fix-Stain protocol.

经过测试的应用

Positive Single Cell (Intra) detected in10x Genomics Gene Expression Flex with Feature Barcodes and Multiplexing product.

推荐稀释比

ApplicationDilution
SINGLE CELL (INTRA)<0.5ug/test
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

G14418-1-5C targets ATP1A1 in applications and shows reactivity with Human samples.

Tested Applications Single Cell (Intra)
Tested Reactivity Human
Immunogen ATP1A1 fusion protein Ag5763 种属同源性预测
Host / Isotype Rabbit / IgG
Class Oligo Conjugate
Type Polyclonal
Full Name MultiProTM 5CFLX Anti-Human ATP1A1 (Polyclonal)
Synonyms ATP1A1, Na(+)/K(+) ATPase 1, Na(+)/K(+) ATPase alpha 1 subunit, Na+/K+ ATPase 1, Sodium potassium ATPase alpha 1 polypeptide, Sodium pump subunit alpha 1
Calculated Molecular Weight 113 kDa
GenBank Accession NumberBC050359
Gene Symbol ATP1A1
Gene ID (NCBI) 476
ENSEMBL Gene IDENSG00000163399
Conjugate 5CFLX
Full Oligo SequenceCGGAGATGTGTATAAGAGACAGGAACATAATGATCCTCCCATATAAGAAA
Barcode SequenceGAACATAATGATCCT
Form Liquid
UNIPROT IDP05023
Storage Buffer PBS with 1mM EDTA and 0.09% sodium azide
Storage Conditions2-8°C

背景介绍

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. For optimal WB detection of this membrane protein, we recommend to avoid boiling the sample after lysis.

Protocols

Our current MultiPro staining protocol for the cocktailDownload protocol
10x Genomics CG000149_Demonstrated Protocol CellSurface Protein Labeling_Rev D.pdfDownload protocol