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CoraLite®594-conjugated NDP52 Monoclonal antibody

NDP52 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2F2C2

Cat No : CL594-66401

Print datasheet

Synonyms

CALCOCO2, NDP52, Nuclear domain 10 protein 52, Nuclear dot protein 52

验证数据展示

  • FC experiment of Jurkat using CL594-66401

    FC experiment of Jurkat using CL594-66401

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human NDP52 (CL594-66401, Clone:2F2C2) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL594-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 66401-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66401 targets NDP52 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra)
Tested Reactivity human
Immunogen NDP52 fusion protein Ag2866 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name calcium binding and coiled-coil domain 2
Synonyms CALCOCO2, NDP52, Nuclear domain 10 protein 52, Nuclear dot protein 52
Calculated Molecular Weight 446 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC015893
Gene Symbol CALCOCO2
Gene ID (NCBI) 10241
RRIDAB_2919986
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13137
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

NDP52, also named as CALCOCO2, is an autophagy receptor. It plays a role in ruffle formation and actin cytoskeleton organization. Mouse/Rat NDP52 has some isoforms with MW 28-40 kDa and 67 kDa. Human NDP52 has some isoforms with MW 43-47 kDa and 52-55 kDa,

实验方案

Product Specific Protocols
FC protocol for CL594 NDP52 antibody CL594-66401Download protocol
Standard Protocols
Click here to view our Standard Protocols