• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Nestin Polyclonal antibody

Nestin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat No : 30760-1-AP

Print datasheet

Synonyms

FLJ21841, Nbla00170, NES, nestin

验证数据展示

  • WB analysis using 30760-1-AP

    WB analysis using 30760-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30760-1-AP (Nestin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 30760-1-AP

    IHC staining of rat brain using 30760-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30760-1-AP (Nestin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 30760-1-AP

    IHC staining of rat brain using 30760-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30760-1-AP (Nestin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 30760-1-AP

    IHC staining of mouse brain using 30760-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30760-1-AP (Nestin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 30760-1-AP

    IHC staining of mouse brain using 30760-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30760-1-AP (Nestin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inSGC-7901 cells, SH-SY5Y cells
Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30760-1-AP targets Nestin in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA
Tested Reactivity Human
Immunogen Nestin fusion protein Ag29600 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name nestin
Synonyms FLJ21841, Nbla00170, NES, nestin
Calculated Molecular Weight 177 kDa
Observed Molecular Weight240-280 kDa
GenBank Accession NumberNM_006617
Gene Symbol NES
Gene ID (NCBI) 10763
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP48681
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Nestin, also named as NES and Nbla00170, is a class VI intermediate filament protein expressed in stem cells of the central nervous system (CNS) but not in mature CNS cells. Its expression is used extensively as a marker for neural lineage cells derived from human embryonic stem (hES) and induced pluripotent stem (iPS) cells. Nestin is also expressed in non-neural stem cell populations such as pancreatic islet progenitors and hematopoietic progenitors. Nestin can be detected as 240-280 kDa. Studies point toward nestin heavy posttranslational modifications as possibly serving particular functions in podocytes and in a few other particular cell types(PMID: 17210924).

实验方案

Product Specific Protocols
WB protocol for Nestin antibody 30760-1-APDownload protocol
IHC protocol for Nestin antibody 30760-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols