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GNAS Polyclonal antibody 5 Publications

Rabbit Polyclonal| Catalog number: 10150-2-AP

Featured Product KD/KO validated

浓度: 170 μg/ml

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Applications

Tested Applications:
IHC, IP, WB, ELISA
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Cited Applications:
IF, WB


Images See all

WB analysis of mouse liver using 10150-2-AP

mouse liver tissue were subjected to SDS PAGE followed by western blot with 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

IP experiment of mouse liver using 10150-2-AP

IP Result of anti-GNAS (IP:10150-2-AP, 4ug; Detection:10150-2-AP 1:300) with mouse liver tissue lysate 4000ug.

IHC staining of human pancreas using 10150-2-AP

Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 10x lens).

IHC staining of human pancreas using 10150-2-AP

Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 40x lens).

Species specificity:
human, mouse
Positive WB detected in:
mouse liver tissue
Positive IP detected in:
mouse liver tissue
Positive IHC detected in:
human pancreas tissue, human colon tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:20-1:200

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
GNAS fusion protein Ag0197
Full name:
GNAS complex locus
Calculated molecular weight:
45 kDa
Observed molecular weight:
46 kDa
GenBank accession number:
BC002722
Gene ID (NCBI):
2778
Gene symbol
GNAS
Synonyms
AHO, C20orf45, GNAS, GNAS complex locus, GNAS1, GPSA, GSA, GSP, NESP, PHP1A, PHP1B, POH

Background

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.

Protocols

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