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  • Featured Product
  • KD/KO Validated

NEU3 Polyclonal antibody

NEU3 Polyclonal Antibody for IF-P, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

IF-P, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27879-1-AP

Print datasheet

Synonyms

cDNA FLJ78026, NEU3, NEU3 protein, SIAL3

验证数据展示

  • WB analysis using 27879-1-AP

    WB analysis using 27879-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27879-1-AP (NEU3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse testis using 27879-1-AP

    IHC staining of mouse testis using 27879-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 27879-1-AP (NEU3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse small intestine using 27879-1-AP

    IF Staining of mouse small intestine using 27879-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using NEU3 antibody (27879-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHEK-293T cells, HL-60 cells, Jurkat cells, K-562 cells, THP-1 cells
Positive IHC detected inmouse testis tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse small intestine tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27879-1-AP targets NEU3 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IF-P, IHC, WB, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity Human, mouse
Cited Reactivityhuman, mouse
Immunogen NEU3 fusion protein Ag27259 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sialidase 3 (membrane sialidase)
Synonyms cDNA FLJ78026, NEU3, NEU3 protein, SIAL3
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 48, 52 kDa
GenBank Accession NumberNM_006656.5
Gene Symbol NEU3
Gene ID (NCBI) 10825
RRIDAB_2881002
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UQ49
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for NEU3 antibody 27879-1-APDownload protocol
IHC protocol for NEU3 antibody 27879-1-APDownload protocol
IF protocol for NEU3 antibody 27879-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

JCI Insight

The mRNA binding protein DDX3 mediates TGF-β1 upregulation of translation and promotes pulmonary fibrosis

Authors - Wensheng Chen
View Article
human,mouseWB,IHC

Food Funct

Polyphenol from millet bran increases the sensitivity of colorectal cancer cells to oxaliplatin by blocking the ganglioside GM3 catabolism.

Authors - Xiaoli Zhang
  • KD Validated
View Article
mouseIF

Exp Lung Res

Changes in lung sialidases in male and female mice after bleomycin aspiration

Authors - Darrell Pilling
View Article