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NMDAR1 Polyclonal antibody

NMDAR1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 27232-1-AP

Print datasheet

Synonyms

GluN1, GRIN1, NMDAR1, NR1

验证数据展示

  • WB analysis using 27232-1-AP

    WB analysis using 27232-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27232-1-AP (NMDAR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of rat brain using 27232-1-AP

    IP experiment of rat brain using 27232-1-AP

    IP result of anti-NMDAR1 (IP:27232-1-AP, 4ug; Detection:27232-1-AP 1:400) with rat brain tissue lysate 1120 ug.

  • IHC staining of mouse brain using 27232-1-AP

    IHC staining of mouse brain using 27232-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27232-1-AP (NMDAR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat brain using 27232-1-AP

    IF Staining of rat brain using 27232-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NMDAR1 antibody (27232-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of rat brain using 27232-1-AP

    IF Staining of rat brain using 27232-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using NMDAR1 antibody (27232-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inrat brain tissue
Positive IHC detected inmouse brain tissue, mouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27232-1-AP targets NMDAR1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen NMDAR1 fusion protein Ag26093 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name glutamate receptor, ionotropic, N-methyl D-aspartate 1
Synonyms GluN1, GRIN1, NMDAR1, NR1
Calculated Molecular Weight 105 kDa
Observed Molecular Weight116-120 kDa
GenBank Accession NumberNM_000832
Gene Symbol GRIN1
Gene ID (NCBI) 2902
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ05586
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRIN1 encodes subunit 1 of the N-methyl-D-aspartate (NMDA) receptor, which is a heteromeric glutamate-gated calcium ion channel essential for synaptic function in the brain (PMID: 25864721, PMID: 25864721). NMDARs play important roles in normal brain development and function, such as synaptic plasticity, neural development, learning and memory (PMID: 20716669). NMDAR dysfunction has been associated with several neurological disorders including Parkinson, Alzheimer and Huntington diseases. Disrupted motor learning and long-term synaptic plasticity in mice lacking NMDAR1 in the striatum (PMID: 17015831).

实验方案

Product Specific Protocols
WB protocol for NMDAR1 antibody 27232-1-APDownload protocol
IHC protocol for NMDAR1 antibody 27232-1-APDownload protocol
IF protocol for NMDAR1 antibody 27232-1-APDownload protocol
IP protocol for NMDAR1 antibody 27232-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols