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  • Featured Product
  • KD/KO Validated

NUAK2 Polyclonal antibody

NUAK2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11592-1-AP

Print datasheet

Synonyms

NUAK family SNF1 like kinase 2, NUAK2, SNARK, SNF1/AMP kinase related kinase

验证数据展示

  • WB analysis of Y79 using 11592-1-AP

    WB analysis of Y79 using 11592-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 11592-1-AP (NUAK2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 11592-1-AP

    IHC staining of human kidney using 11592-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11592-1-AP (NUAK2 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 11592-1-AP

    IHC staining of human kidney using 11592-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 11592-1-AP (NUAK2 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of NIH3T3 cells using 11592-1-AP

    IF Staining of NIH3T3 cells using 11592-1-AP

    NIH3T3 cells on cover slips fixed and staining with anti-NUAK2 (11592-1-AP,1:100;Rabbit polyclonal -Red) and anti-pericentrin(centrosome Marker; McAb-Green). The photo shows the centrosome in cell cycle: Interphase, Prophase, Prometaphase, Metaphase, Anaphase and Telophase. DAPI was used to stain the cell nuclei (blue) at a concentration of 1ug/ml.(from Dr. Zineldeen Doaa Hussein) .

经过测试的应用

Positive WB detected inY79 cells, HeLa cells
Positive IHC detected inhuman kidney tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11592-1-AP targets NUAK2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen NUAK2 fusion protein Ag2031 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name NUAK family, SNF1-like kinase, 2
Synonyms NUAK family SNF1 like kinase 2, NUAK2, SNARK, SNF1/AMP kinase related kinase
Calculated Molecular Weight 628 aa, 70 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC017306
Gene Symbol NUAK2
Gene ID (NCBI) 81788
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H093
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NUAK2(NUAK family SNF1-like kinase 2) is also named as OMPHK2, SNARK and belongs to the SNF1 subfamily. The function of NUAK2 is partly dependent on anti-apoptotic properties against apoptosis induced by death ligand and NUAK2 also has effects on the migration of cancer cells as speculated from studies on myosin filaments and cytoskeleton organization in normal cells(PMID:21911917). Western blot analysis detected NUAK2 expressed in cancer cell lines of diverse tissue origin(PMID:12409306).

实验方案

Product Specific Protocols
WB protocol for NUAK2 antibody 11592-1-APDownload protocol
IHC protocol for NUAK2 antibody 11592-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Proc Natl Acad Sci U S A

AMP kinase-related kinase NUAK2 affects tumor growth, migration, and clinical outcome of human melanoma.

Authors - Namiki Takeshi T
View Article
humanWB, IF

Cancer Res

NUAK2 amplification coupled with PTEN deficiency promote melanoma development via CDK activation.

Authors - Takeshi Namiki
  • KD Validated
View Article
humanWB

Diabetologia

Regulation of skeletal muscle sucrose, non-fermenting 1/AMP-activated protein kinase-related kinase (SNARK) by metabolic stress and diabetes.

Authors - Rune A A
  • KD Validated
View Article
humanWB, IF

Ann Clin Biochem

Sucrose non-fermenting AMPK related kinase/Pentraxin 3 and DNA damage axis: a gateway to cardiovascular disease in systemic lupus erythematosus among Egyptian patients.

Authors - Doaa Hussein Zineldeen
View Article